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四季草莓(Fragaria x ananassa Duch.)连续结果性状的渐渗与验证

Introgression and confirmation of everbearing trait in strawberry ( x Duch.).

作者信息

Negi Shivanti, Sharma Girish, Sharma Rajnish

机构信息

Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173 230 India.

Department of Fruit Science, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173 230 India.

出版信息

Physiol Mol Biol Plants. 2020 Dec;26(12):2407-2416. doi: 10.1007/s12298-020-00916-w. Epub 2020 Dec 6.

Abstract

UNLABELLED

The present investigation was targeted towards a highly desirable everbearing trait in strawberry ( ×  Duch.) via marker assisted selection while seeing its worldwide commercial applicability through the extended harvest season. The crosses were made between everbearing and june-bearing cultivars to raise the F individuals. Morphological characters (plant, floral, and fruit) were assessed that showed significant differences among the strawberry cultivars. Molecular characterization was carried out between everbearing and non-everbearing cultivars using RAPD and SSR markers. For phenotyping, a chi-square test was performed and revealed that out of all four cross combinations, the best fitted cross found to be in Mendelian segregation ratio (1:1) was 'Confectura' × 'Torrey' with χ-value 1.58. Further, the identified polymorphic markers were assessed across the F individuals of cross 'Confectura' × 'Torrey' for its genotyping. It could be revealed that the targeted everbearing trait is governed by a dominant gene(s) in the subjected strawberry genotypes. Further, the identified polymorphic markers would be successfully employed in DNA fingerprinting of strawberry under various crop improvement programme.

SUPPLEMENTARY INFORMATION

The online version of this article (10.1007/s12298-020-00916-w) contains supplementary material, which is available to authorized users.

摘要

未标注

本研究旨在通过标记辅助选择培育草莓(× 杜氏)中极具吸引力的四季结果性状,同时通过延长收获季节来考察其在全球的商业适用性。以四季结果品种和一季结果品种进行杂交,培育出F个体。对形态特征(植株、花和果实)进行了评估,结果表明草莓品种间存在显著差异。利用RAPD和SSR标记对四季结果品种和非四季结果品种进行了分子鉴定。对于表型分析,进行了卡方检验,结果显示在所有四种杂交组合中,最符合孟德尔分离比(1:1)的杂交组合是‘Confectura’בTorrey’,卡方值为1.58。此外,对杂交组合‘Confectura’בTorrey’的F个体进行了已鉴定多态性标记的基因分型评估。结果表明,在受试草莓基因型中,目标四季结果性状由一个显性基因控制。此外,已鉴定的多态性标记将成功应用于各种作物改良计划下的草莓DNA指纹分析。

补充信息

本文的在线版本(10.1007/s12298-020-00916-w)包含补充材料,授权用户可获取。

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