Department of Dermatology and Venereology, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia.
Department of Dermatology and Venereology, Faculty of Medicine, University of Indonesia, Depok 16424, West Java, Indonesia.
Pan Afr Med J. 2020 Oct 13;37:145. doi: 10.11604/pamj.2020.37.145.15213. eCollection 2020.
this research aimed to analyze nerve growth factor (NGF) contents as diagnostic tools for early disability in leprosy patients and the cut-off point value.
research samples consisted of 79 leprosy patients with disability grade 0 or 1 who met the clinically approved inclusion criteria. The age of patients ranged from 14 to 50 years. For both sample groups, blood serum was collected to determine NGF concentration. NGF level was analyzed by enzyme-linked immunosorbent assay (ELISA) according to the manual guide of the kit insert from Cussabio®. Statistical analysis used SPSS 17 software for Windows. A comparison was performed with the Student's t-test and the NGF concentration cut-off point was determined using a receiver operating characteristic (ROC) curve.
the research result demonstrated that NGF concentration in multibacillary leprosy with disability grade 0 was higher than in grade 1. Leprosy with disability grade 0 had an NGF content reaching 100.46 pg/mL, while those with grade 1 had a lower concentration of NGF at 30.56 pg/mL. The higher disability grade indicated a lower NGF concentration in the blood serum. Based on the ROC analysis result, the NGF cut-off was shown to be 81.43 pg/mL. This result indicated that low NGF in nerve and skin lesions of leprosy patients contributes to early peripheral nerve malfunction due to Mycobacterium leprae infection.
these results prove that NGF can be used as a marker of early disability in leprosy, with the cut-off value at 81.43 pg/mL.
本研究旨在分析神经生长因子（NGF）含量作为麻风病患者早期残疾的诊断工具及其截断值。
研究样本包括 79 名残疾等级为 0 或 1 的麻风病患者，符合临床批准的纳入标准。患者年龄在 14 至 50 岁之间。对两组样本均采集血清，以确定 NGF 浓度。NGF 水平通过酶联免疫吸附试验（ELISA）分析，试剂盒采用 Cussabio®的说明书进行操作。统计分析使用 Windows 版 SPSS 17 软件。采用学生 t 检验进行比较，并使用受试者工作特征（ROC）曲线确定 NGF 浓度截断值。
研究结果表明，具有残疾等级 0 的多菌型麻风病患者的 NGF 浓度高于等级 1。具有残疾等级 0 的麻风病患者的 NGF 含量达到 100.46pg/mL，而具有等级 1 的患者的 NGF 浓度较低，为 30.56pg/mL。残疾等级越高，血清中的 NGF 浓度越低。根据 ROC 分析结果，NGF 的截断值为 81.43pg/mL。这一结果表明，麻风病患者神经和皮肤损伤中 NGF 含量降低，导致麻风分枝杆菌感染引起的早期外周神经功能障碍。
这些结果证明，NGF 可作为麻风病早期残疾的标志物，截断值为 81.43pg/mL。
