College of Environmental Science and Engineering, Zhejiang Gongshang University, No.18 Xuezheng Street, Hangzhou, Zhejiang Province, China.
Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Islamabad, Pakistan.
Biodegradation. 2021 Feb;32(1):73-85. doi: 10.1007/s10532-021-09926-1. Epub 2021 Jan 13.
The effect of electricity, induced by external resistance, on microbial community performance is investigated in Microbial Fuel Cells (MFCs) involved in simultaneous biotransformation of sulfide and nitrate. In the experiment, three MFCs were operated under different external resistances (100 Ω, 1000 Ω and 10,000 Ω), while one MFC was operated with open circuit as control. All MFCs demonstrate good capacity for simultaneous sulfide and nitrate biotransformation regardless of external resistance. MFCs present similar voltage profile; however, the output voltage has positive relationship with external resistance, and the MFC1 with lowest external resistance (100 Ω) generated highest power density. High-throughput sequencing confirms that taxonomic distribution of suspended sludge in anode chamber encompass phylum level to genus level, while the results of principal component analysis (PCA) suggest that microbial communities are varied with external resistance, which external resistance caused the change of electricity generation and substrate removal at the same, and then leads to the change of microbial communities. However, based on Pearson correlation analyses, no strong correlation is evident between community diversity indices (ACE index, Chao index, Shannon index and Simpson index) and the electricity (final voltage and current density). It is inferred that the performance of electricity did not significantly affect the diversity of microbial communities in MFCs biotransforming sulfide and nitrate simultaneously.
在外阻诱导的电流作用下，研究了参与同时转化硫化物和硝酸盐的微生物燃料电池（MFC）中微生物群落性能的变化。实验中，三个 MFC 在不同的外阻（100 Ω、1000 Ω 和 10,000 Ω）下运行，一个 MFC 作为对照采用开路运行。所有 MFC 均表现出良好的同时转化硫化物和硝酸盐的能力，而与外阻无关。MFC 呈现出相似的电压曲线；然而，输出电压与外阻呈正相关，外阻最低（100 Ω）的 MFC1 产生的功率密度最高。高通量测序证实，阳极室悬浮污泥的分类分布涵盖了门水平到属水平，而主成分分析（PCA）的结果表明，微生物群落随外阻而变化，外阻导致发电和基质去除的变化同时发生，进而导致微生物群落的变化。然而，基于 Pearson 相关分析，群落多样性指数（ACE 指数、Chao 指数、Shannon 指数和 Simpson 指数）与电（最终电压和电流密度）之间没有明显的强相关性。可以推断，电的性能并没有显著影响同时转化硫化物和硝酸盐的 MFC 中微生物群落的多样性。
