Sertoli 细胞表达基因 secernin-1（Scrn1）在小鼠中对雄性生育力可有可无。
The Sertoli cell expressed gene secernin-1 (Scrn1) is dispensable for male fertility in the mouse.
机构信息
School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
出版信息
Dev Dyn. 2021 Jul;250(7):922-931. doi: 10.1002/dvdy.299. Epub 2021 Jan 25.
BACKGROUND
Male infertility is a prevalent clinical presentation for which there is likely a strong genetic component due to the thousands of genes required for spermatogenesis. Within this study we investigated the role of the gene Scrn1 in male fertility. Scrn1 is preferentially expressed in XY gonads during the period of sex determination and in adult Sertoli cells based on single cell RNA sequencing. We investigated the expression of Scrn1 in juvenile and adult tissues and generated a knockout mouse model to test its role in male fertility.
RESULTS
Scrn1 was expressed at all ages examined in the post-natal testis; however, its expression peaked at postnatal days 7-14 and SCRN1 protein was clearly localized to Sertoli cells. Scrn1 deletion was achieved via removal of exon 3, and its loss had no effect on male fertility or sex determination. Knockout mice were capable of siring litters of equal size to wild type counterparts and generated equal numbers of sperm with comparable motility and morphology characteristics.
CONCLUSIONS
Scrn1 was found to be dispensable for male fertility, but this study identifies SCRN1 as a novel marker of the Sertoli cell cytoplasm.
背景
男性不育是一种常见的临床病症，由于精子发生需要数千个基因，因此可能存在很强的遗传因素。在这项研究中，我们研究了基因 Scrn1 在男性生育能力中的作用。Scrn1 在性别决定期间的 XY 性腺中优先表达，并根据单细胞 RNA 测序在成年支持细胞中表达。我们研究了 Scrn1 在幼年和成年组织中的表达，并生成了敲除小鼠模型以测试其在男性生育能力中的作用。
结果
Scrn1 在出生后的睾丸中所有检查的年龄均有表达；然而，其表达在出生后第 7-14 天达到峰值，SCRN1 蛋白明显定位于支持细胞。通过去除外显子 3 实现 Scrn1 缺失，其缺失对男性生育力或性别决定没有影响。敲除小鼠能够与野生型对照一样产生同等大小的后代，并产生具有可比运动和形态特征的同等数量的精子。
结论
发现 Scrn1 对于男性生育能力不是必需的，但本研究确定 SCRN1 为支持细胞细胞质的新型标记物。
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