Zhang Ying, Jia Shuaijun, Tian Fang, Gao Xinlin, Wang Zhiyuan, Zhu Lei, Hao Dingjun
Shaanxi University of Traditional Chinese Medicine, Xianyang Shaanxi, 712046, P.R.China.
Department of Spine Surgery, Honghui Hospital Affiliated to Medical School of Xi'an Jiaotong University, Xi'an Shaanxi, 710054, P.R.China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2021 Jan 15;35(1):104-110. doi: 10.7507/1002-1892.202007088.
After using hyaluronic acid (HA) to modify curcumin (CUR), the effects of calcium phosphate cement (CPC) combined with HA/CUR on the proliferation and osteogenesis of osteoblasts were investigated.
First, HA and CUR were esterified and covalently combined to prepare HA/CUR, and the characteristics were observed and the infrared spectrum was tested. Then, HA, CUR, and HA/CUR were mixed with CPC according to 5% ( / ) to prepare HA-CPC, CUR-CPC, and HA/CUR-CPC, respectively. Setting time detection, scanning electron microscope observation, injectable performance test, and compression strength test were conducted; and the CPC was used as a control. Osteoblasts were isolated and cultured from the skull of newborn Sprague Dawley rats, and the 2nd generation cells were cultured with the 4 types of bone cement, respectively. The effects of HA/CUR-CPC on the proliferation and osteogenesis of osteoblasts were estimated by the scanning electron microscopy observation, live/dead cell fluorescence staining, cell counting, osteopontin (OPN) immunofluorescence staining, alkaline phosphatase (ALP) staining,and alizarin red staining.
Infrared spectroscopy test showed that HA and CUR successfully covalently combined. The HA/CUR-CPC group had no significant difference in initial setting time, final setting time, injectable rate, and compressive strength when compared with the other 3 groups ( >0.05); scanning electron microscope observation showed that HA/CUR was scattered on CPC surface. After co-culture of bone cement and osteoblasts, scanning electron microscopy observation showed that the osteoblasts, which had normal morphology and the growth characteristics of osteoblasts, clustered and adhered to HA/CUR-CPC. There was no significant difference in cell survival rate between HA/CUR-CPC group and other groups ( >0.05), and the number of cells significantly increased ( <0.05); the degrees of OPN immunofluorescence staining, ALP staining, and alizarin red staining were stronger than other groups.
HA/CUR-CPC has good biocompatibility and mechanical properties, which can promote the proliferation and osteogenesis of osteoblasts.
用透明质酸（HA）修饰姜黄素（CUR）后，研究磷酸钙骨水泥（CPC）复合HA/CUR对成骨细胞增殖和成骨的影响。
首先，将HA与CUR进行酯化及共价结合制备HA/CUR，观察其特性并检测红外光谱。然后，将HA、CUR和HA/CUR分别按5%（/）与CPC混合，制备HA-CPC、CUR-CPC和HA/CUR-CPC，进行凝结时间检测、扫描电子显微镜观察、可注射性能测试及抗压强度测试，以CPC作为对照。从新生Sprague Dawley大鼠颅骨分离培养成骨细胞，将第2代细胞分别与4种骨水泥培养，通过扫描电子显微镜观察、活/死细胞荧光染色、细胞计数、骨桥蛋白（OPN）免疫荧光染色、碱性磷酸酶（ALP）染色及茜素红染色评估HA/CUR-CPC对成骨细胞增殖和成骨的影响。
红外光谱检测显示HA与CUR成功共价结合。HA/CUR-CPC组与其他3组相比，初凝时间、终凝时间、可注射率及抗压强度差异无统计学意义（>0.05）；扫描电子显微镜观察显示HA/CUR散在分布于CPC表面。骨水泥与成骨细胞共培养后，扫描电子显微镜观察显示成骨细胞形态正常，具有成骨细胞生长特性，聚集并黏附于HA/CUR-CPC。HA/CUR-CPC组与其他组细胞存活率差异无统计学意义（>0.05），细胞数量显著增加（<0.05）；OPN免疫荧光染色、ALP染色及茜素红染色程度均强于其他组。
HA/CUR-CPC具有良好的生物相容性和力学性能，可促进成骨细胞增殖和成骨。
