Akel Bilgiç Hayriye, Gürel Şeref Can, Ayhan Yavuz, Karahan Sevilay, Karakaya İbrahim, Babaoğlu Melih, Dilbaz Nesrin, Uluğ Berna Diclenur, Demir Başaran, Karaaslan Çağatay
Turk Psikiyatri Derg. 2020 Winter;31(4):232-238. doi: 10.5080/u25044.
Neuropeptide Y (NPY) is a protein widely expressed in the central nervous system and involved in diverse physiological processes, such as emotional regulation, nutritional behavior, and stress. In some populations, studies on alcohol dependence (AD) and the NPY gene have found that NPY variations increase alcohol consumption and thus may potentially be associated with AD. In this study, we investigated the relationship between NPY gene promoter polymorphisms and phenotypes related to alcohol use.
A total of 417 male participants comprising 252 individuals with AD and 165 healthy individuals were included in this study and phenotypic data were collected. Polymerase chain reaction-restriction fragment length polymorphism (PCR/RFLP) and DNA sequencing METHODS were used for genotyping the rs16147 and rs17149106 polymorphisms in the promoter region of the NPY gene. The data of 384 participants were analysed to evaluate the possible relationship between genotypes and the diagnosis of AD, family history of AD, the severity of AD using the Michigan Alcoholism Screening Test (MAST), the age of onset of problematic alcohol use, the average amount of alcohol consumed per day for the last six months and the lifetime maximum alcohol consumption in one day.
A significant difference was found between the AD and control groups concerning rs16147 polymorphism genotype distribution (p=0.025). No association with polymorphisms and alcohol-related phenotypes were demonstrated in the AD group.
To our knowledge, this study shows for the first time in the literature that alcohol dependence is associated with NPY rs16147 polymorphism in the Turkish population.
神经肽Y（NPY）是一种在中枢神经系统广泛表达的蛋白质，参与多种生理过程，如情绪调节、营养行为和应激反应。在一些人群中，关于酒精依赖（AD）与NPY基因的研究发现，NPY变异会增加酒精摄入量，因此可能与AD相关。在本研究中，我们调查了NPY基因启动子多态性与酒精使用相关表型之间的关系。
本研究共纳入417名男性参与者，其中252名患有AD，165名健康个体，并收集了表型数据。采用聚合酶链反应-限制性片段长度多态性（PCR/RFLP）和DNA测序方法对NPY基因启动子区域的rs16147和rs17149106多态性进行基因分型。对384名参与者的数据进行分析，以评估基因型与AD诊断、AD家族史、使用密歇根酒精ism筛查测试（MAST）评估的AD严重程度、问题性酒精使用的发病年龄、过去六个月每天的平均酒精摄入量以及一生中一天的最大酒精摄入量之间的可能关系。
在rs16147多态性基因型分布方面，AD组与对照组之间存在显著差异（p=0.025）。在AD组中未发现多态性与酒精相关表型之间的关联。
据我们所知，本研究首次在文献中表明，在土耳其人群中，酒精依赖与NPY rs16147多态性相关。
