BrzeziŃska Sylwia Agata, Bielecka Teresa, Zabost Anna, GŁogowska Agnieszka, KoziŃska Monika, Augustynowicz-KopeĆ Ewa
Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland.
Department of Pediatric Pulmonology and Allergy, Medical University of Warsaw, Warsaw, Poland.
Cent Eur J Immunol. 2020;45(2):130-135. doi: 10.5114/ceji.2020.97900. Epub 2020 Jul 27.
Bacillus Calmette-Guérin (BCG) is the only tuberculosis vaccine available and although it has been routinely used for more than 80 years, its protective effect varies depending on the age and the form of tuberculosis. Due to the close analogy between the vaccine strain and other species of the Mycobacterium tuberculosis complex, molecular methods are recommended for differential diagnosis of post-BCG complications. The aim of the study was to assess usefulness of molecular methods in diagnosis of post-BCG vaccine adverse events (VAEs).
M. tuberculosis complex strains obtained in 2011-2017 from 68 ill children were subjected to molecular analysis.
Molecular analysis of 68 strains showed 100% agreement between the results in the GenoType MTBC method and the multiplex PCR method. For the strains isolated from 45 patients with suspected VAE, M. bovis BCG was obtained, whereas the strains isolated from the remaining 23 children were identified as M. tuberculosis. The analysis confirmed the close relationship between the result of identification and the type of material as well as the patient's age.
The use of genetic methods enables quick and detailed diagnostics of infections caused by M. bovis BCG, which allows for the confirmation or exclusion of VAE.
卡介苗（BCG）是唯一可用的结核病疫苗，尽管它已被常规使用80多年，但其保护效果因结核病的年龄和形式而异。由于疫苗株与结核分枝杆菌复合群的其他物种之间存在密切的相似性，因此推荐使用分子方法对卡介苗接种后的并发症进行鉴别诊断。本研究的目的是评估分子方法在诊断卡介苗接种后不良事件（VAE）中的实用性。
对2011年至2017年从68名患病儿童中获得的结核分枝杆菌复合群菌株进行分子分析。
对68株菌株的分子分析表明，GenoType MTBC方法和多重PCR方法的结果100%一致。从45例疑似VAE患者中分离出的菌株为牛型结核分枝杆菌卡介苗，而从其余23名儿童中分离出的菌株被鉴定为结核分枝杆菌。分析证实了鉴定结果与材料类型以及患者年龄之间的密切关系。
使用基因方法能够快速、详细地诊断由牛型结核分枝杆菌卡介苗引起的感染，从而确认或排除VAE。
