The development of analytical methods for the purity determination of fucoidan extracted from brown seaweed species.

To determine the purity of extracted fucoidan from brown seaweeds, analytical methods were developed, including spectroscopy (i.e., Attenuate total reflectance (ATR) - Fourier-transform infrared (FT-IR) and Raman) combined with chemometrics; and the results were compared with those of high performance liquid chromatography (HPLC) and other two chemistry methods (i.e., fucoidan estimation based on fucose content and a cationic dye method based on sulphated polysaccharide estimation). Quantitative models (i.e., partial least squares regression (PLSR)) were developed and cross-validated using FT-IR spectroscopic methods (RCV ~ 0.998, RMSECV ~1.7%). The models were also validated using other four commercial fucoidan products. On the other hand, the same commercial samples were used to validate the two chemistry methods and the HPLC method. Estimation results of these analytical methods were discussed based on the potential of these analytical methods for fucoidan purity determination. The results demonstrated FT-IR spectroscopy with chemometrics potentially could be used for non-destructive and real time determination.