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用于植物组织切片表型特征分析的多元拉曼映射

Multivariate Raman mapping for phenotypic characterization in plant tissue sections.

作者信息

Liedtke Ingrid, Diehn Sabrina, Heiner Zsuzsanna, Seifert Stephan, Obenaus Sabine, Büttner Carmen, Kneipp Janina

机构信息

Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany.

Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany; School of Analytical Sciences Adlershof SALSA, Humboldt-Universität zu Berlin, Albert-Einstein-Straße 5-11, 12489 Berlin, Germany.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119418. doi: 10.1016/j.saa.2020.119418. Epub 2021 Jan 2.

Abstract

Identifying and characterizing the biochemical variation in plant tissues is an important task in many research fields. Small spectral differences of the plant cell wall that are caused by genetic or environmental influences may be superimposed by individual variation as well as by a microscopic heterogeneity in molecular composition and structure of different histological substructures. A set of 56 samples from Cucumis sativus (cucumber) plants, comprising a total of ~168,000 spectra from tissue sections of leaf, stem, and roots was investigated by Raman microspectroscopic mapping excited at 532 nm. A multivariate analysis was carried out in order to assess the variation of the spectra with respect to origin of the tissue, the histological (cell wall) substructures, and the possibility to discriminate the spectra obtained from different individuals that had been subjected to two different conditions during growth. Combining the results of principal component analysis (PCA) based classification with the original spatial information in the maps of 23 sections of leaf xylem, variation in cell wall composition is found for four different individuals that also includes a discrimination of tissue grown in the presence and absence of additional silicic acid in the irrigation water of the plants. The spectral data point to differences in a contribution by carotenoids, as well as by hydroxycinnamic acids to the spectra. The results give new insight into the chemical heterogeneity of plant tissues and may be useful for elucidating biochemical processes associated with biomineralization by vibrational spectroscopy.

摘要

识别和表征植物组织中的生化变化是许多研究领域的一项重要任务。由遗传或环境影响引起的植物细胞壁微小光谱差异,可能会被个体差异以及不同组织亚结构分子组成和结构的微观异质性所叠加。通过对532nm激发的拉曼显微光谱成像,研究了一组来自黄瓜植株的56个样本,这些样本总共包含来自叶、茎和根组织切片的约168,000个光谱。进行了多变量分析,以评估光谱相对于组织来源、组织学(细胞壁)亚结构的变化,以及区分从生长过程中经历两种不同条件的不同个体获得的光谱的可能性。将基于主成分分析(PCA)的分类结果与23个叶木质部切片图谱中的原始空间信息相结合,发现了四个不同个体的细胞壁组成变化,其中还包括对在植物灌溉水中添加和不添加硅酸的情况下生长的组织的区分。光谱数据表明类胡萝卜素以及羟基肉桂酸对光谱的贡献存在差异。这些结果为植物组织的化学异质性提供了新的见解,可能有助于通过振动光谱阐明与生物矿化相关的生化过程。

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