通过金@聚多巴胺壳纳米探针在活的人类癌细胞中对致癌性微小RNA进行长期检测
Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine-Shell Nanoprobe.
作者信息
Wong Wai Ki, Wong Siu Hong Dexter, Bian Liming
机构信息
Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, 999077 China.
Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, 518172 China.
出版信息
ACS Biomater Sci Eng. 2020 Jul 13;6(7):3778-3783. doi: 10.1021/acsbiomaterials.0c00633. Epub 2020 Jun 19.
Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines . A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.
致癌性微小RNA（miRNA），例如miR-155，是癌细胞中驱动肿瘤发生的关键肿瘤生物标志物，并且miRNA谱特征可以预测癌症的发展和侵袭性。因此，及时检测活细胞中的致癌性miRNA对癌症的早期诊断极具吸引力。在此，我们报道了一种基于金@聚多巴胺的高度序列特异性纳米探针，用于长期检测人癌细胞系中的miRNA。单次施用该纳米探针能够连续检测活癌细胞中miR-155的表达水平长达5天。我们相信我们的纳米探针在肿瘤学研究和转化应用方面都极具前景。
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