Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan.
Innovation and Development Center of Sustainable Agriculture (IDCSA), National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.
J Agric Food Chem. 2021 Jan 27;69(3):913-921. doi: 10.1021/acs.jafc.0c05628. Epub 2021 Jan 19.
Although bacteria with 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity have been used to mitigate biotic and abiotic stresses in crops, it is not well known whether the ACC deaminase gene () in is related to the alleviation of salt stress by the bacterium. This study aimed to evaluate the effects of in strain CHB 1107 on the nutrient uptake and growth of tomato plants under salt stress. The mutant (CHB 1107 M) of CHB 1107 was obtained through bacterial conjugation. Wild-type (CHB 1107 WT) and CHB 1107 M were used to inoculate tomato plants grown in a soil or solution with an electrical conductivity of 6 dS/m adjusted by NaCl. CHB 1107 M completely lost the ability to produce ACC deaminase, whereas the complementation of in CHB 1107 M preserved its ACC deaminase activity. CHB 1107 WT significantly reduced the production of ethylene and proline by tomato plants under salt stress, increasing the shoot and root dry weights of tomato plants compared with the noninoculated control and CHB 1107 M. In addition, tomato plants inoculated with CHB 1107 M showed a significant reduction in K (27.5%), Ca (23.0%), and Mn uptake (17.5%) compared with those inoculated with CHB 1107 WT. In contrast, CHB 1107 WT significantly reduced Na uptake by tomato plants in comparison to CHB 1107 M in saline soil conditions. In addition, the inoculation of tomato plants with CHB 1107 WT resulted in a higher K/Na ratio than in those inoculated with CHB 1107 M and the noninoculated control. These findings suggest that in is associated with the amelioration of salinity stress in tomato. Plant transformation with and the field application of may be used as potential management tools for crops under salt stress.
尽管具有 1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性的细菌已被用于减轻作物的生物和非生物胁迫，但尚不清楚 是否的 ACC 脱氨酶基因 () 与该细菌缓解盐胁迫有关。本研究旨在评估 在 菌株 CHB 1107 中对盐胁迫下番茄植株养分吸收和生长的影响。 通过细菌接合获得 的 突变体 (CHB 1107 M)。野生型 (CHB 1107 WT) 和 CHB 1107 M 用于接种在电导率为 6 dS/m 的土壤或溶液中生长的番茄植株，该电导率通过 NaCl 进行调节。CHB 1107 M 完全丧失了产生 ACC 脱氨酶的能力，而在 CHB 1107 M 中 的互补保留了其 ACC 脱氨酶活性。与未接种对照和 CHB 1107 M 相比，CHB 1107 WT 显著降低了盐胁迫下番茄植株的乙烯和脯氨酸的产生，增加了番茄植株的地上部和根干重。此外，与接种 CHB 1107 WT 的番茄植株相比，接种 CHB 1107 M 的番茄植株对 K（27.5%）、Ca（23.0%）和 Mn 的吸收量分别减少了 27.5%、23.0%和 17.5%。相比之下，在盐渍土壤条件下，与 CHB 1107 M 相比，CHB 1107 WT 显著降低了番茄植株对 Na 的吸收。此外，与接种 CHB 1107 M 和未接种对照的番茄植株相比，接种 CHB 1107 WT 的番茄植株的 K/Na 比值更高。这些发现表明 在 中与番茄盐胁迫的缓解有关。通过 和 的植物转化以及 在田间的应用可以作为盐胁迫下作物的潜在管理工具。
