Jiang Hanfang, Meng Qing, Liu Xiaorong, Chen Hongyu, Zhu Chunqing, Chen Yunsheng
Clinical Laboratory, Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen, Guangdong, People's Republic of China.
Clinical Laboratory, Shenzhen Children's Hospital, Shenzhen, Guangdong, People's Republic of China.
Infect Drug Resist. 2021 Jan 11;14:49-58. doi: 10.2147/IDR.S286187. eCollection 2021.
To determine the phenotypes and genotypes of invasive (), 108 strains were isolated from paediatric patients with invasive pneumococcal diseases (IPDs) in Shenzhen from 2014 to 2018.
Serotype profiles were defined by multiplex PCR of the capsule gene. Pneumococcal surface protein A (PspA) classification was performed through gene sequencing. Antimicrobial resistance was examined by broth microdilution. Multilocus sequence typing (MLST) was determined based on next-generation sequencing data.
Eighty-one of 17 serotypes were finally collected. The coverage of the 13-conjugated polysaccharide vaccine (PCV13) was 88.9%. After the introduction of PCV13, the nonvaccine serotypes were added by serotypes 15b, 16F and 20. Vaccine serotype 3 increased by four serious cases. The pspA family 1 and pspA family 2 are predominant. The multiple drug resistance rate is 91.3%. None of the nonmeningitis isolates were resistant to penicillin, while 98.8% of all the isolates were resistant to erythromycin.
This work characterizes the molecular epidemiology of invasive in Shenzhen. Continued surveillance of serotype distribution and antimicrobial susceptibility is necessary to alert antibiotic-resistant nonvaccine serotypes and highly virulent serotypes.
为确定侵袭性肺炎球菌的表型和基因型，从2014年至2018年期间深圳的小儿侵袭性肺炎球菌疾病（IPD）患者中分离出108株菌株。
通过对荚膜基因进行多重PCR来确定血清型谱。通过基因测序进行肺炎球菌表面蛋白A（PspA）分类。采用肉汤微量稀释法检测抗菌药物耐药性。基于下一代测序数据确定多位点序列分型（MLST）。
最终收集到17种血清型中的81种。13价结合多糖疫苗（PCV13）的覆盖率为88.9%。引入PCV13后，非疫苗血清型增加了15B、16F和20型。疫苗血清型3增加了4例重症病例。PspA家族1和PspA家族2占主导地位。多重耐药率为91.3%。所有非脑膜炎分离株均对青霉素不耐药，而所有分离株中有98.8%对红霉素耐药。
本研究描述了深圳侵袭性肺炎球菌的分子流行病学特征。持续监测血清型分布和抗菌药物敏感性对于警惕耐抗生素的非疫苗血清型和高毒力血清型很有必要。
