双隐性甜蜡质玉米的淀粉物理化学性质。
Starch physicochemical properties of double recessive sweet-waxy maize.
机构信息
Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Key Laboratory of Crop Cultivation and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou 225009, China.
Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
出版信息
Int J Biol Macromol. 2021 Mar 15;173:219-224. doi: 10.1016/j.ijbiomac.2021.01.116. Epub 2021 Jan 19.
Sweet-waxy is a new type of maize with waxy and sugary double recessive genes. This study aims to clarify starch structural and functional properties of this maize type. Grains with sweet-waxy and waxy phenotypes were separated from an ear using the two sweet-waxy maize hybrids of ATN and NKY as materials. Compared with waxy maize starch, the sweet-waxy maize starch mainly comprises small-sized round granules despite the typical waxy character of both starches. Mw, Mn, and relative crystallinity of sweet-waxy starch were higher than those of waxy starch in both hybrids. The average chain length of waxy starch was higher in ATN but lower in NKY compared with that of sweet-waxy starch. However, polydispersity (Mw/Mn) and F1 fraction were high in sweet-waxy and waxy starches in ATN and NKY, respectively. Breakdown viscosity, gelatinization enthalpy and temperatures of both hybrids were low in sweet-waxy starch. Peak viscosity was higher in waxy starch in NKY and similar between sweet-waxy and waxy starches in ATN. Retrogradation percentage was high and low for sweet-waxy starches in ATN and NKY, respectively.
甜蜡质是一种具有蜡质和甜质双隐性基因的新型玉米。本研究旨在阐明这种玉米类型的淀粉结构和功能特性。以 ATN 和 NKY 两个甜蜡质玉米杂种为材料，从一个穗上分离出甜蜡质和蜡质两种表型的籽粒。与蜡质玉米淀粉相比，尽管两种淀粉都具有典型的蜡质特征，但甜蜡质玉米淀粉主要由小而圆的颗粒组成。在两个杂种中，甜蜡质淀粉的Mw、Mn 和相对结晶度均高于蜡质淀粉。与甜蜡质淀粉相比，ATN 中的蜡质淀粉的平均链长较高，而 NKY 中的则较低。然而，在 ATN 和 NKY 中，甜蜡质和蜡质淀粉的多分散性（Mw/Mn）和 F1 分数均较高。两种杂交种的破裂粘度、糊化焓和温度均较低，在 NKY 中，蜡质淀粉的峰值粘度较高，而在 ATN 中，甜蜡质和蜡质淀粉的峰值粘度相似。在 ATN 中，甜蜡质淀粉的回生百分比较高，而在 NKY 中则较低。
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