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SARS-CoV-2 诱导恒河猴生发中心 CD4 T 滤泡辅助细胞产生强烈反应。

SARS-CoV-2 induces robust germinal center CD4 T follicular helper cell responses in rhesus macaques.

机构信息

Center for Immunology and Infectious Diseases, UC Davis, Davis, CA, USA.

Graduate Group in Immunology, UC Davis, Davis, CA, USA.

出版信息

Nat Commun. 2021 Jan 22;12(1):541. doi: 10.1038/s41467-020-20642-x.

DOI:10.1038/s41467-020-20642-x
PMID:33483492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7822826/
Abstract

CD4 T follicular helper (T) cells are important for the generation of durable and specific humoral protection against viral infections. The degree to which SARS-CoV-2 infection generates T cells and stimulates the germinal center (GC) response is an important question as we investigate vaccine induced immunity against COVID-19. Here, we report that SARS-CoV-2 infection in rhesus macaques, either infused with convalescent plasma, normal plasma, or receiving no infusion, resulted in transient accumulation of pro-inflammatory monocytes and proliferating T cells with a T1 profile in peripheral blood. CD4 helper cell responses skewed predominantly toward a T1 response in blood, lung, and lymph nodes. SARS-CoV-2 Infection induced GC T cells specific for the SARS-CoV-2 spike and nucleocapsid proteins, and a corresponding early appearance of antiviral serum IgG antibodies. Collectively, the data show induction of GC responses in a rhesus model of mild COVID-19.

摘要

CD4 T 滤泡辅助（T）细胞对于产生针对病毒感染的持久和特异性体液保护非常重要。我们研究针对 COVID-19 的疫苗诱导免疫时，一个重要的问题是 SARS-CoV-2 感染产生的 T 细胞数量以及刺激生发中心（GC）反应的程度。在这里，我们报告在恒河猴中，SARS-CoV-2 感染无论是输注恢复期血浆、正常血浆还是未输注，都会导致外周血中促炎单核细胞和增殖 T 细胞的短暂积累，T1 表型。CD4 辅助细胞反应在血液、肺和淋巴结中主要偏向 T1 反应。SARS-CoV-2 感染诱导针对 SARS-CoV-2 刺突蛋白和核衣壳蛋白的 GC T 细胞，以及抗病毒血清 IgG 抗体的早期出现。总的来说，这些数据表明在轻度 COVID-19 的恒河猴模型中诱导了 GC 反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/9eb06a7971bc/41467_2020_20642_Fig10_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/9bd785e502f9/41467_2020_20642_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/1ac319978f41/41467_2020_20642_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/9eb06a7971bc/41467_2020_20642_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/2c60f6d51cc6/41467_2020_20642_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/890ba8d73b40/41467_2020_20642_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/0410aac97599/41467_2020_20642_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/02abbd887aac/41467_2020_20642_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/cbe4565f94b5/41467_2020_20642_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/14b180b63b33/41467_2020_20642_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/39a86e53dc05/41467_2020_20642_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/9bd785e502f9/41467_2020_20642_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/1ac319978f41/41467_2020_20642_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2c9/7822826/9eb06a7971bc/41467_2020_20642_Fig10_HTML.jpg

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