Macau Institute for Applied Research in Medicine and Health, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau.
Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macau.
Food Chem. 2021 Jun 15;347:129008. doi: 10.1016/j.foodchem.2021.129008. Epub 2021 Jan 7.
Probiotics can release many bioactive peptides that confer a myriad of benefits to the host health. However, exploring new bioactive peptides secreted by probiotics is hampered by lots of matrix-related interference peptides from the medium, and the low abundance. To this end, a new approach integrating mixed-mode cationic exchange based solid-phase extraction (MCX-SPE) coupled with liquid chromatography-mass spectrometry (LC-MS) and feature-based molecular networking (FBMN) was developed. FBMN's intuitive visualization results enabled twenty-five novel peptides to be quickly discovered and characterized from the cultures of three strains of Bifidobacterium, B. animalis, B. longum, and B. pseudolongum. Interestingly, four were uniquely secreted by B. animalis treated with gypenosides, and one showed ACE inhibitory effect with an IC value of 193.22 μM. Consequently, this approach could serve as a powerful tool for quickly discovering bioactive peptides from the complex metabolites of probiotics, which contributes to the development of functional foods and nutraceuticals.
益生菌可以释放许多具有生物活性的肽，从而为宿主健康带来多种益处。然而，由于培养基中存在大量基质相关干扰肽和低丰度的问题，探索益生菌分泌的新生物活性肽受到了阻碍。为此，本研究开发了一种新的方法，将基于混合模式阳离子交换的固相萃取（MCX-SPE）与液相色谱-质谱（LC-MS）和基于特征的分子网络（FBMN）相结合。FBMN 的直观可视化结果使得能够从三株双歧杆菌（Bifidobacterium），即动物双歧杆菌（B. animalis）、长双歧杆菌（B. longum）和假长双歧杆菌（B. pseudolongum）的培养物中快速发现和鉴定出 25 种新型肽。有趣的是，有 4 种肽是由用绞股蓝皂苷处理的动物双歧杆菌特异性分泌的，其中一种具有 ACE 抑制活性，IC 值为 193.22 μM。因此，该方法可以作为一种从益生菌复杂代谢产物中快速发现生物活性肽的有力工具，有助于功能性食品和营养保健品的开发。
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