Division of Pulmonary and Critical Care Medicine, Oregon Health and Science University, Portland, OR, USA.
Methods Mol Biol. 2021;2241:161-181. doi: 10.1007/978-1-0716-1095-4_14.
Eosinophils affect nerve structure and function in organs such as lungs and skin, which contributes to disease pathogenesis. We have developed methods for culturing primary sensory and parasympathetic neurons in multiple species and have refined these techniques for coculture with eosinophils. Eosinophil-nerve coculture has been an essential tool for testing interactions between these cell types. Here we describe methods for coculturing primary parasympathetic ganglia, vagal sensory nerves, and dorsal root sensory nerves with eosinophils.
嗜酸性粒细胞影响肺和皮肤等器官的神经结构和功能，从而导致疾病的发生。我们已经开发出在多种物种中培养原代感觉神经元和副交感神经元的方法，并对这些技术进行了改进，以用于与嗜酸性粒细胞共培养。嗜酸性粒细胞-神经共培养是测试这些细胞类型之间相互作用的重要工具。在这里，我们描述了与嗜酸性粒细胞共培养副交感神经节、迷走感觉神经和背根感觉神经的方法。
