Dongre P M, Jaiswal Vinod D, Singh Suraj
Department of Biophysics, University of Mumbai, Mumbai, Maharashtra, India.
J Med Phys. 2020 Jul-Sep;45(3):187-194. doi: 10.4103/jmp.JMP_89_19. Epub 2020 Oct 13.
Biophysical study to investigate (a) the effects of smartphone light fluxes (SPLF) on isolated mammalian cornea and model protein (insulin), (b) to predict the possible visual interference of SPLF.
Fresh goat cornea and insulin protein were used as an experimental model system. The energy of absorbed SPLF was measured using chemical dosimeter. The effect of SPLF on the aggregation of model protein was studied using fluorescence spectroscopy and dynamic light scattering (DLS). Fluorescence microscopy, scanning electron microscopy (SEM), DLS, were used for cornea imaging.
The spectral emission peak of SPLF was observed at 380 nm and 420 nm. Absorbed radiation of SPLF was found to be 2.82 mWm and 1.92 mWm for collimated (focussed) and noncollimated (nonfocussed) condition, respectively. Secondary structural changes of insulin were observed by fluorescence and zeta potential after SPLF exposure. SEM study revealed the disorganization of the epithelial cell surface, increase in intercellular space, disorganization of primary epithelium layer, and exposure of the second layer is seen in depth. Differential Interference Microscopy showed an optical gradient in images that appears to be changed in specimen structure. Fluorescence microscopy showed disorganization in epithelial cell pattern. A significant difference in bio-molecular permeation was observed in the exposed cornea. Ultraviolet UV-visible spectroscopy study indicated a reduction in light transmission through the cornea.
The obtained results indicate changes in physicochemical and morphological modifications in the cornea and insulin modifications after exposed to SPLF.
进行生物物理研究，以调查（a）智能手机光通量（SPLF）对离体哺乳动物角膜和模型蛋白（胰岛素）的影响，（b）预测SPLF可能产生的视觉干扰。
使用新鲜山羊角膜和胰岛素蛋白作为实验模型系统。使用化学剂量计测量吸收的SPLF能量。使用荧光光谱法和动态光散射（DLS）研究SPLF对模型蛋白聚集的影响。荧光显微镜、扫描电子显微镜（SEM）、DLS用于角膜成像。
观察到SPLF的光谱发射峰在380nm和420nm处。在准直（聚焦）和非准直（非聚焦）条件下，SPLF的吸收辐射分别为2.82mW/m²和1.92mW/m²。暴露于SPLF后，通过荧光和zeta电位观察到胰岛素二级结构的变化。SEM研究显示上皮细胞表面紊乱、细胞间隙增加、初级上皮层紊乱，并且在深度上可见第二层暴露。微分干涉显微镜显示图像中的光学梯度似乎在标本结构中发生了变化。荧光显微镜显示上皮细胞模式紊乱。在暴露的角膜中观察到生物分子渗透有显著差异。紫外可见光谱研究表明通过角膜的光透射率降低。
所得结果表明，暴露于SPLF后，角膜的物理化学和形态学发生了变化，胰岛素也发生了改变。
