Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekis avenue 7, LT-10257 Vilnius, Lithuania.
Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekis avenue 7, LT-10257 Vilnius, Lithuania.
Int J Biol Macromol. 2021 Mar 15;173:421-434. doi: 10.1016/j.ijbiomac.2021.01.133. Epub 2021 Jan 22.
In this study lipolytic biocatalysts GD-95RM, GDEst-95 and GDEst-lip were immobilized by encapsulation in calcium alginate beads All three immobilized biocatalysts demonstrated significantly increased thermal stability at 60-70 °C temperatures and the activity of GD-95RM lipase increased by 50% at 70-80 °C following the immobilization. Moreover, encapsulated GDEst-95 esterase retained higher than 50% lipolytic activity after 3 months of incubation with butanol (25%) and ethanol (50%); GDEst-lip enzyme possessed 50% activity after 2 months of treatment with ethanol (25%) and methanol (25%); and GD-95RM lipase displayed higher that 50% activity after two-week incubation with methanol (50%). All three immobilized enzymes displayed long-term storage capability (>50% activity) at least until 3 months at 4 °C. It was also detected that immobilized GD-95RM and GDEst-lip can perform flow hydrolysis of both avocado oil and p-NP dodecanoate in prototype packed-bed column reactor. The analysis of continuous transesterification of avocado or sunflower oil with ethanol or methanol as substrates confirmed that encapsulated GD-95RM and GDEst-lip enzymes is a useful approach to produce fatty acid alkyl esters.
在这项研究中，脂解生物催化剂 GD-95RM、GDEst-95 和 GDEst-lip 通过包埋在海藻酸钠珠中进行固定化。三种固定化生物催化剂在 60-70°C 的温度下表现出显著提高的热稳定性，并且在固定化后，GD-95RM 脂肪酶的活性在 70-80°C 下提高了 50%。此外，包埋的 GDEst-95 酯酶在与丁醇（25%）和乙醇（50%）孵育 3 个月后保留了高于 50%的脂解活性；GDEst-lip 酶在乙醇（25%）和甲醇（25%）处理 2 个月后具有 50%的活性；而 GD-95RM 脂肪酶在甲醇（50%）孵育两周后显示出高于 50%的活性。三种固定化酶在 4°C 下至少 3 个月都具有长期储存能力（>50%的活性）。还检测到固定化的 GD-95RM 和 GDEst-lip 可以在原型填充床柱反应器中对鳄梨油和 p-NP 月桂酸酯进行连续水解。对鳄梨油或葵花油与乙醇或甲醇作为底物的连续酯交换的分析证实，包埋的 GD-95RM 和 GDEst-lip 酶是生产脂肪酸烷基酯的有效方法。
