Steele Joel R, Italiano Carly J, Phillips Connor R, Violi Jake P, Pu Lisa, Rodgers Kenneth J, Padula Matthew P
Proteomics Core Facility and School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia.
Neurotoxin Research Group, School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia.
Proteomes. 2021 Jan 21;9(1):2. doi: 10.3390/proteomes9010002.
Proteinopathies are diseases caused by factors that affect proteoform conformation. As such, a prevalent hypothesis is that the misincorporation of noncanonical amino acids into a proteoform results in detrimental structures. However, this hypothesis is missing proteomic evidence, specifically the detection of a noncanonical amino acid in a peptide sequence. This review aims to outline the current state of technology that can be used to investigate mistranslations and misincorporations whilst framing the pursuit as Misincorporation Proteomics (MiP). The current availability of technologies explored herein is mass spectrometry, sample enrichment/preparation, data analysis techniques, and the hyphenation of approaches. While many of these technologies show potential, our review reveals a need for further development and refinement of approaches is still required.
蛋白质病是由影响蛋白质异构体构象的因素引起的疾病。因此，一个普遍的假说是，非标准氨基酸错误掺入蛋白质异构体会导致有害结构。然而，这一假说缺乏蛋白质组学证据，特别是在肽序列中检测到非标准氨基酸。本综述旨在概述可用于研究错误翻译和错误掺入的当前技术状态，同时将这一研究方向界定为错误掺入蛋白质组学（MiP）。本文探讨的当前可用技术包括质谱、样品富集/制备、数据分析技术以及方法的联用。虽然这些技术中的许多都显示出了潜力，但我们的综述表明，仍需要进一步开发和完善这些方法。
