College of Chemistry, Chemical Engineering and Material Science, Soochow University, Suzhou, 215123, People's Republic of China.
First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.
Mikrochim Acta. 2021 Jan 26;188(2):53. doi: 10.1007/s00604-021-04710-7.
In order to effectively and conveniently detect the β-amyloid oligomer (AβO) for earlier diagnosis of Alzheimer's disease (AD), a disposable aptamer biosensor has been developed with high performance, facile operation, and low cost. Using a nanocomposite by in situ reduction of chloroauric acid to decorate Au nanoparticles (AuNPs) on Fe-MIL-88NH material via Au-N bond to effectively enhance the electrochemiluminescence (ECL) of luminol, the functioned basal electrode provides adequate background for sensing response. When the aptamer is linked via Au-S bond on the surface, the sensor gets the ability of specific recognition and coalescence toward the target (AβO). After incubating the sample on the aptasensor, its ECL signal is inhibited owing to the steric hindrance of the AβO macromolecules. The relative inhibition ratio linearly depends to the logarithm of AβO concentration in the range 0.1 pM to 10 pM, with an LOD of 71 fM. The aptasensor has high selectivity to AβO among its analogs. The recoveries in human serum were 98.9-105.4%. This research provides a new approach for sensitive detection of AβO in clinic laboratories for investigation and diagnosis of AD.
为了有效且便捷地检测β-淀粉样寡聚体（AβO），以便对阿尔茨海默病（AD）进行早期诊断，我们研发了一种具有高性能、操作简便和低成本等特点的一次性适体生物传感器。该传感器通过原位还原氯金酸，在 Fe-MIL-88NH 材料上修饰金纳米粒子（AuNPs），通过 Au-N 键有效增强了鲁米诺的电化学发光（ECL）效应，功能化的基底电极提供了充足的感应响应背景。当适体通过 Au-S 键连接到表面时，传感器获得了针对目标（AβO）的特异性识别和聚集能力。将样品孵育在适体传感器上后，由于 AβO 大分子的空间位阻，其 ECL 信号受到抑制。相对抑制率与 AβO 浓度的对数在 0.1 pM 至 10 pM 的范围内呈线性依赖关系，检出限为 71 fM。该适体传感器对 AβO 及其类似物具有高选择性。在人血清中的回收率为 98.9-105.4%。这项研究为在临床实验室中对 AβO 进行灵敏检测提供了一种新方法，可用于 AD 的研究和诊断。
