Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People's Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Fuzhou, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People's Republic of China, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, China.
Plant Biol (Stuttg). 2021 May;23(3):517-527. doi: 10.1111/plb.13238. Epub 2021 Mar 24.
Imbibitional chilling stress inhibits normal seed germination and seedling establishment and leads to large losses in peanut production. This is a major limiting factor when sowing peanut earlier and further north. To reveal the response mechanism of peanut to imbibitional chilling stress, a Tandem Mass Tag (TMT)-based quantitative proteomics analysis was conducted to identify differentially accumulated proteins (DAPs) under imbibitional chilling stress. Hormone profiling and transcriptional analysis were performed to confirm the proteomics data. Further seed priming analysis with exogenous cytokinins was conducted to validate the role of cytokinins in alleviating imbibitional chilling injury. A total of 5029 proteins were identified and quantified in all of the experimental groups. Among these, 104 proteins were DAPs as compared with the control. Enrichment analysis revealed that these DAPs were significant in various molecular functional and biological processes, especially for biosynthesis and metabolism of plant hormones. Hormone profiling and transcription analysis suggested that the reduced abundance of cytokinin oxidase may be caused by down-regulation of gene expression of the corresponding genes and leads to an elevated content of cytokinins under chilling stress. Seed priming analysis suggested that exogenous application of cytokinins may alleviate injury caused by imbibitional chilling. Our study provides a comprehensive proteomics analysis of peanut under imbibitional chilling stress, suggesting the role of plant hormones in the response mechanism. The results provide a better understanding of the imbibitional chilling stress response mechanism in peanut that will aid in peanut production.
吸水冷胁迫抑制正常种子萌发和幼苗建立，导致花生产量的巨大损失。这是在更早和更北的地方播种花生的主要限制因素。为了揭示花生对吸水冷胁迫的响应机制，进行了基于串联质量标签（TMT）的定量蛋白质组学分析，以鉴定吸水冷胁迫下差异积累的蛋白质（DAPs）。进行激素分析和转录分析以验证蛋白质组学数据。进一步用外源细胞分裂素进行种子引发分析，以验证细胞分裂素在缓解吸水冷胁迫损伤中的作用。在所有实验组中总共鉴定和定量了 5029 种蛋白质。其中，与对照相比，有 104 种蛋白质是 DAPs。富集分析表明，这些 DAPs 在各种分子功能和生物过程中显著，特别是在植物激素的生物合成和代谢中。激素分析和转录分析表明，细胞分裂素氧化酶的丰度降低可能是由于相应基因表达的下调，导致冷胁迫下细胞分裂素含量升高。种子引发分析表明，外源施用细胞分裂素可缓解吸水冷胁迫造成的伤害。我们的研究对花生吸水冷胁迫下进行了全面的蛋白质组学分析，表明植物激素在响应机制中的作用。研究结果为花生吸水冷胁迫响应机制提供了更好的理解，有助于花生生产。
