Virology and Biosafety Laboratories Unit, "Lazzaro Spallanzani"-IRCCS, National Institute for Infectious Diseases, 00149 Rome, Italy.
Laboratory of Clinical Pathology, San Camillo-Forlanini Hospital, 00149 Rome, Italy.
Viruses. 2021 Jan 25;13(2):176. doi: 10.3390/v13020176.
Molecular investigation of primary HIV infections (PHI) is crucial to describe current dynamics of HIV transmission. Aim of the study was to investigate HIV transmission clusters (TC) in PHI referred during the years 2013-2020 to the National Institute for Infectious Diseases in Rome (INMI), that is the Lazio regional AIDS reference centre, and factors possibly associated with inclusion in TC. These were identified by phylogenetic analysis, based on population sequencing of ; a more in depth analysis was performed on TC of B subtype, using ultra-deep sequencing (UDS) of . Of 270 patients diagnosed with PHI during the study period, 229 were enrolled (median follow-up 168 (IQR 96-232) weeks). Median age: 39 (IQR 32-48) years; 94.8% males, 86.5% Italians, 83.4% MSM, 56.8% carrying HIV-1 subtype B. Of them, 92.6% started early treatment within a median of 4 (IQR 2-7) days after diagnosis; median time to sustained suppression was 20 (IQR 8-32) weeks. Twenty TC (median size 3, range 2-9 individuals), including 68 patients, were identified. A diagnosis prior to 2015 was the unique factor associated with inclusion in a TC. Added value of UDS was the identification of shared quasispecies components in transmission pairs within TC.
对原发性 HIV 感染 (PHI) 进行分子研究对于描述 HIV 传播的当前动态至关重要。本研究的目的是调查 2013 年至 2020 年期间提交给罗马国家传染病研究所 (INMI) 的 PHI 中的 HIV 传播簇 (TC)，该研究所是拉齐奥地区艾滋病参考中心，以及可能与 TC 纳入相关的因素。这些因素是通过基于 的群体测序进行系统发育分析确定的;对 B 亚型的 TC 进行了更深入的分析，使用 的超深度测序 (UDS)。在研究期间，共诊断出 270 例 PHI 患者，其中 229 例入组 (中位随访 168 (IQR 96-232) 周)。中位年龄：39 (IQR 32-48) 岁;94.8%男性，86.5%意大利人，83.4%男男性接触者，56.8%携带 HIV-1 亚型 B。其中，92.6%在诊断后中位数为 4 (IQR 2-7) 天内开始早期治疗;中位持续抑制时间为 20 (IQR 8-32) 周。确定了 20 个 TC (中位数大小为 3，范围为 2-9 人)，包括 68 名患者。2015 年前的诊断是唯一与 TC 纳入相关的因素。UDS 的附加价值是在 TC 内的传播对中识别共享准种成分。
