Deviche P, Moore F L
Oregon State University, Department of Zoology, Corvallis 97331.
Horm Behav. 1988 Mar;22(1):26-34. doi: 10.1016/0018-506x(88)90028-1.
Adult, sexually mature, male rough-skinned newts (Taricha granulosa) obtained from a wild population were castrated and received Silastic capsules containing testosterone (T), estradiol (E), or 5 alpha-dihydrotestosterone (DHT). The newts received three capsules of T, either one or three capsules of E or DHT, or combined treatments with these two steroids. When tested for sexual responsiveness after 32 and 34 days of steroid treatment, no group differed from the castrated controls (C). After 74 and 75 days of treatment, more T-implanted than C newts were sexually responsive, but the newts treated with E, DHT, or these two steroids in combination did not differ behaviorally from the C group. The diameter of the vas deferens was greater in the T- and DHT-treated males than in the C males, indicating that the implants adequately replaced testicular androgens. Together with other studies on this and other species, these results confirm the participation of testosterone in the regulation of sexual behaviors in male amphibians. Furthermore, these results indicate that in this amphibian, the behavioral effects of T are mediated directly by this steroid, not indirectly by enzymatic conversion to DHT or E.
从野生种群中获取成年、性成熟的雄性粗糙皮肤蝾螈（Taricha granulosa），对其进行阉割，并植入含有睾酮（T）、雌二醇（E）或5α - 双氢睾酮（DHT）的硅橡胶胶囊。蝾螈接受了三粒T胶囊、一粒或三粒E或DHT胶囊，或这两种类固醇的联合治疗。在类固醇治疗32天和34天后测试性反应时，没有一组与阉割对照组（C）有差异。治疗74天和75天后，植入T的蝾螈比C组蝾螈有更多的性反应，但用E、DHT或这两种类固醇联合治疗的蝾螈在行为上与C组没有差异。接受T和DHT治疗的雄性蝾螈的输精管直径比C组雄性蝾螈的大，表明植入物充分替代了睾丸雄激素。与关于该物种和其他物种的其他研究一起，这些结果证实了睾酮参与雄性两栖动物性行为的调节。此外，这些结果表明，在这种两栖动物中，T的行为效应是由这种类固醇直接介导的，而不是通过酶促转化为DHT或E间接介导的。
