乙型肝炎肝硬化患者中幽门螺杆菌与肝功能、炎症因子及 FoxP3、RORγt 血清水平的相关性。

Correlations of Helicobacter pylori with liver function, inflammatory factors and serum levels of FoxP3 and RORγt in patients with hepatitis B cirrhosis.

机构信息

Department of Infectious Diseases, Shengjing Hospital, Affiliated Hospital of China Medical University, Shenyang, China.

出版信息

Eur Rev Med Pharmacol Sci. 2021 Jan;25(1):459-465. doi: 10.26355/eurrev_202101_24415.

DOI:10.26355/eurrev_202101_24415

PMID:33506937

Abstract

OBJECTIVE

To investigate the correlations of Helicobacter pylori (HP) with liver function, inflammatory factors and serum levels of forkhead box P3 (FoxP3) and retinoic acid receptor-related orphan receptor gamma-t (RORγt) in patients with hepatitis B cirrhosis (HBC).

PATIENTS AND METHODS

A total of 60 HBC patients were divided into HBC group (n=30) and HP-infected HBC group (HP&HBC group, n=30). QRT-PCR was conducted to determine the messenger ribonucleic acid (mRNA) levels of FoxP3 and RORγt in serum samples. ELISA was applied to measure the levels of relevant inflammatory factors. Besides, immunohistochemical staining was conducted to detect positive expressions of FoxP3 and RORγt in liver tissues of patients in the two groups.

RESULTS

No significant differences in gender, drinking, smoking, diabetes and age were found between HBC group and HP&HBC group (p>0.05). Globulin and albumin levels were comparable between the two groups (p>0.05). Liver function indexes, including ALT, AST and TBIL were higher in HP&HBC group than those in HBC group (p<0.05). The HBV-DNA level was lower in HBC group in comparison with that in HP&HBC group. The interferon-gamma (IFN-γ) level was remarkably higher in HBC group than that in HP&HBC group (p<0.01), and the levels of interleukin (IL)-6, IL-10, IL-17 and transforming growth factor (TGF)-β1 were notably lower in HBC group in comparison with those in HP&HBC group (p<0.01). Additionally, the mRNA levels of FoxP3 and RORγt in HBC group were distinctly lower than those in HP&HBC group (p<0.01). The mRNA levels of FoxP3 and RORγt were positively related to those of IL-6, IL-10, IL-17, and TGF-β1, and negatively associated with IFN-γ level. Immunohistochemical results indicated that positive expression rates of FoxP3 and RORγt in the liver tissues were approximately 50% in HP&HBC group and B. Zhao, Q.-J. Sheng, Y. Qin, X.-L. Wang, H. Zhao, N. Zhaowere 15% in HBC group, and the difference was statistically significant (p<0.05).

CONCLUSIONS

Expression levels of FoxP3 and RORγt in serum and liver tissues are elevated in HP-infected HBC patients, and inflammatory factors are correlated with their expressions, suggesting the aggravated liver damage.

摘要

目的

研究乙型肝炎肝硬化（HBC）患者中幽门螺杆菌（HP）与肝功能、炎症因子及叉头框 P3（FoxP3）和维甲酸相关孤儿受体γt（RORγt）血清水平的相关性。

患者和方法

将 60 例 HBC 患者分为 HBC 组（n=30）和 HP 感染 HBC 组（HP&HBC 组，n=30）。采用实时荧光定量聚合酶链反应（qRT-PCR）检测血清样本中 FoxP3 和 RORγt 的信使核糖核酸（mRNA）水平。采用酶联免疫吸附试验（ELISA）测定相关炎症因子水平。此外，采用免疫组化染色法检测两组患者肝组织中 FoxP3 和 RORγt 的阳性表达。

结果

HBC 组和 HP&HBC 组在性别、饮酒、吸烟、糖尿病和年龄方面无显著差异（p>0.05）。两组球蛋白和白蛋白水平相当（p>0.05）。HP&HBC 组丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和总胆红素（TBIL）等肝功能指标均高于 HBC 组（p<0.05）。HBC 组 HBV-DNA 水平低于 HP&HBC 组。HBC 组干扰素-γ（IFN-γ）水平明显高于 HP&HBC 组（p<0.01），HBC 组白细胞介素（IL）-6、IL-10、IL-17 和转化生长因子（TGF）-β1 水平明显低于 HP&HBC 组（p<0.01）。此外，HBC 组 FoxP3 和 RORγt 的 mRNA 水平明显低于 HP&HBC 组（p<0.01）。FoxP3 和 RORγt 的 mRNA 水平与 IL-6、IL-10、IL-17 和 TGF-β1 的水平呈正相关，与 IFN-γ水平呈负相关。免疫组化结果表明，HP&HBC 组肝组织 FoxP3 和 RORγt 的阳性表达率约为 50%，HBC 组为 15%，差异有统计学意义（p<0.05）。