Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria.
Laboratory for Reproductive Biology and Developmental Programming, Department of Physiology, Edo University Iyamho, Nigeria.
JBRA Assist Reprod. 2021 Apr 27;25(2):252-256. doi: 10.5935/1518-0557.20200091.
Xylopia aethiopica is a common plant in West Africa, with wide applications in trado-medical management of several diseases. Thus, our study aimed to analyze the histology and hormonal effects of ethanol extracts of Xylopia aethiopica seeds on cadmium chloride-induced reproductive dysfunction in female Wistar rats.
We used twenty-five rats weighing 120-150g for this study. The rats were divided into five groups (n=5). Group 1: received only distilled water orally; Group 2: received 2 mg/kg cadmium chloride orally; Group 3: received 2 mg/kg cadmium chloride plus 50 mg/kg Xylopia aethiopica seeds orally; Group 4: received 2 mg/kg cadmium chloride plus 100 mg/kg Xylopia aethiopica seeds orally, and Group 5: received 100 mg/kg Xylopia aethiopica seeds only, orally. We administered the extracts for 14 days, after which we slaughtered the animals following chloroform anesthesia. We took the blood samples by cardiac puncture for hormonal assay. The ovaries and uterus were harvested for histology. We analyzed the data using ANOVA, and the differences in mean values were considered significant at p<0.05.
The body weight of the rats showed a dose-dependent reduction (p<0.05), compared with the controls. Xylopia aethiopica seeds significantly (p<0.05) reversed the detrimental effects of Cadmium on LH and FSH. The histological analysis of the ovary showed significant improvement upon treatment with Xylopia aethiopica extract in a dose-dependent manner.
The ameliorative effects of Xylopia aethiopica against cadmium chloride-induced reproductive toxicity in female Wistar rats may be attributed to its antioxidant properties.
依桐是一种常见的西非植物，在几种疾病的传统医学治疗中有广泛的应用。因此，我们的研究旨在分析依桐种子乙醇提取物对氯化镉诱导的雌性 Wistar 大鼠生殖功能障碍的组织学和激素作用。
我们使用了 25 只体重为 120-150g 的大鼠进行这项研究。这些大鼠被分为五组（n=5）。第 1 组：仅口服蒸馏水；第 2 组：口服 2mg/kg 氯化镉；第 3 组：口服 2mg/kg 氯化镉加 50mg/kg 依桐种子；第 4 组：口服 2mg/kg 氯化镉加 100mg/kg 依桐种子；第 5 组：仅口服 100mg/kg 依桐种子。我们给予提取物 14 天，然后用氯仿麻醉处死动物。通过心脏穿刺采集血液样本进行激素测定。采集卵巢和子宫进行组织学检查。我们使用方差分析分析数据，平均值的差异在 p<0.05 时被认为有统计学意义。
与对照组相比，大鼠体重呈剂量依赖性下降（p<0.05）。依桐种子显著（p<0.05）逆转了镉对 LH 和 FSH 的有害影响。卵巢的组织学分析显示，依桐提取物以剂量依赖的方式显著改善了卵巢的损伤。
依桐对氯化镉诱导的雌性 Wistar 大鼠生殖毒性的改善作用可能归因于其抗氧化特性。
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