Assessment of the Antileishmanial Potential of Leaf Extract.

has a wide array of biologically active and therapeutically important class of compounds. important drug targets, sterol 24-c methyltransferase (SMT), trypanothione reductase (TR), pteridine reductase (PTR1), and nucleoside hydrolase (NH), were modelled, and molecular docking was performed against the abundant phytochemicals of its leaf extract. Molecular docking results provided the significant prima facie evidence of the leaf extract to have antileishmanial potential. To confirm this, we performed antileishmanial and cytotoxicity assays. Methanolic extract of leaves showed growth inhibition and proliferation of promastigote with an IC value of 43.31 ± 4.202 μg/mL. It also inhibited the growth of intra-macrophagic amastigotes with an IC value of 80.76 ± 3.626 μg/mL. extract was found cytotoxic at a very high concentration on human macrophages (CC = 626 ± 39 μg/mL). Annexin V/propidium iodide (PI) staining assay suggested partial apoptosis induction in parasites by to exert its antileishmanial activity. Here, for the first time, we have shown the antileishmanial potential of leaves. Overall, our results could open new insight for an affordable and natural antileishmanial with high efficacy and less toxicity.