School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; School of Biological and Food Engineering, Chuzhou University, Chuzhou 239000, China.
Ultrason Sonochem. 2021 May;73:105470. doi: 10.1016/j.ultsonch.2021.105470. Epub 2021 Jan 22.
Sugarcane bagasse (SCB) is an abundant agricultural waste in China and the conversion of the waste into plethora of useful resources is very vital. To achieve this, fractionation of the waste is highly important in the biomass biorefinery. The present study aims at investigating the synergistic role of deep eutectic solvents (DES) with sweeping frequency ultrasound (SFUS) and fixed frequency ultrasound (FFUS) in the fractionation of SCB to enhance the enzymatic saccharification process. Therefore, the effects of ultrasound (US) and DES conditions on the pretreatment efficiency were investigated. Under optimum SCB pretreatment conditions, FFUS (40 kHz, 60 min) + DES (choline chloride (ChCl)-lactic acid (LA), 120 °C, 3 h) and SFUS (40 kHz, 60 min) + DES (ChCl-LA, 120 °C, 3 h), the lignin removal rates were 80.13 and 85.62%, respectively. The hemicellulose removal rates were 78.08 and 90.46%, respectively; and the contents of glucose, xylose and arabinose in the liquid fractions after FFUS + DES pretreatment were 7.07, 17.95 and 3.01%, respectively. However, the yield of glucose, xylose, and cellobiose after enzymatic hydrolysis of the SFUS + DES pretreated SCB were 86.76, 38.68, and 20.76%. Analytical studies revealed that the SFUS + DES pretreatment can effectively destroy the ultrastructure of SCB and reduce the crystallinity of cellulose. Furthermore, the mechanism of pretreatment with SFUS + DES was proposed, which confirmed the excellent performance of SFUS + DES. Thus, the application of SFUS + DES pretreatment was able to improve the removal of lignin and hemicellulose from SCBs.
甘蔗渣（SCB）是中国丰富的农业废弃物，将其转化为多种有用资源至关重要。为了实现这一目标，在生物质生物炼制中，对废物进行分级是非常重要的。本研究旨在探讨深共晶溶剂（DES）与扫频超声（SFUS）和固定频率超声（FFUS）协同作用在 SCB 分级中的作用，以提高酶解糖化过程。因此，研究了超声（US）和 DES 条件对预处理效率的影响。在最佳的 SCB 预处理条件下，FFUS（40 kHz，60 min）+DES（氯化胆碱（ChCl）-乳酸（LA），120°C，3 h）和 SFUS（40 kHz，60 min）+DES（ChCl-LA，120°C，3 h），木质素去除率分别为 80.13%和 85.62%；半纤维素去除率分别为 78.08%和 90.46%；FFUS+DES 预处理后液体部分的葡萄糖、木糖和阿拉伯糖含量分别为 7.07%、17.95%和 3.01%。然而，SFUS+DES 预处理 SCB 经酶解后的葡萄糖、木糖和纤维二糖得率分别为 86.76%、38.68%和 20.76%。分析研究表明，SFUS+DES 预处理能有效破坏 SCB 的超微结构，降低纤维素结晶度。此外，还提出了 SFUS+DES 预处理的机制，证实了 SFUS+DES 的优异性能。因此，SFUS+DES 预处理的应用能够提高 SCB 中木质素和半纤维素的去除率。
