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香芹酚,牛至属植物的一种精油成分,可抑制霍乱弧菌的生长和毒力。

Inhibition of growth and virulence of Vibrio cholerae by carvacrol, an essential oil component of Origanum spp.

机构信息

Division of Biochemistry, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

出版信息

J Appl Microbiol. 2021 Sep;131(3):1147-1161. doi: 10.1111/jam.15022. Epub 2021 Feb 22.

Abstract

AIMS

In the age where bacterial resistance to conventional antibiotics is increasing at an alarming rate, the use of the traditional plant, herb extracts or other bioactive constituents is gradually becoming popular as an anti-virulence agent to treat pathogenic diseases. Carvacrol, a major essential oil fraction of Oregano, possesses a wide range of bioactivities. Therefore, we aimed to study the effect of sub-inhibitory concentrations of carvacrol on major virulence traits of Vibrio cholerae.

METHODS AND RESULTS

We have used in vitro as well as ex vivo models to access the anti-pathogenic role of carvacrol. We found that the sub-inhibitory concentration of carvacrol significantly repressed bacterial mucin penetrating ability. Carvacrol also reduced the adherence and fluid accumulation in the rabbit ileal loop model. Reduction in virulence is associated with the downregulated expression of tcpA, ctxB, hlyA and toxT. Furthermore, carvacrol inhibits flagellar synthesis by downregulating the expression of flrC and most of the class III genes.

CONCLUSIONS

Carvacrol exhibited anti-virulence activity against V. cholerae, which involved many events including the inhibition of mucin penetration, adhesion, reduced expression of virulence-associated genes culminating in reduced fluid accumulation.

SIGNIFICANCE AND IMPACT OF THE STUDY

These findings indicate that carvacrol possesses inhibitory activity against V. cholerae pathogenesis and might be considered as a potential bio-active therapeutic alternative to combat cholera.

摘要

目的

在细菌对传统抗生素的耐药性以惊人的速度增长的时代,使用传统的植物、草药提取物或其他生物活性成分作为抗毒剂来治疗致病性疾病逐渐流行起来。香芹酚是牛至的主要精油成分之一,具有广泛的生物活性。因此,我们旨在研究亚抑制浓度的香芹酚对霍乱弧菌主要毒力特性的影响。

方法和结果

我们使用了体外和离体模型来评估香芹酚的抗病原作用。我们发现,亚抑制浓度的香芹酚显著抑制了细菌穿透粘蛋白的能力。香芹酚还减少了兔回肠环模型中的粘附和液体积累。毒力的降低与 tcpA、ctxB、hlyA 和 toxT 的下调表达有关。此外,香芹酚通过下调 flrC 和大多数 III 类基因的表达来抑制鞭毛合成。

结论

香芹酚对霍乱弧菌表现出抗毒力活性,涉及许多事件,包括抑制粘蛋白穿透、粘附、降低与毒力相关基因的表达,最终导致液体积累减少。

研究的意义和影响

这些发现表明,香芹酚对霍乱弧菌的发病机制具有抑制活性,可能被认为是一种潜在的生物活性治疗替代物,用于对抗霍乱。

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