Park Eun Jeong, Myint Phyoe Kyawe, Appiah Michael G, Worawattananutai Patsorn, Inprasit Janjira, Prajuabjinda Onmanee, Soe Zay Yar, Gaowa Arong, Kawamoto Eiji, Shimaoka Motomu
Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
Department of Emergency and Disaster Medicine, Mie University Graduate School of Medicine, Tsu, Mie, 514-8507, Japan.
Biochem Biophys Rep. 2021 Feb 2;26:100932. doi: 10.1016/j.bbrep.2021.100932. eCollection 2021 Jul.
Expression of chemokine receptor CX3CR1 is reportedly restricted to several cell types including natural killer cells, cytotoxic T cells, monocytes, and macrophages. However, its expression and function on exosomes, which are nanosized extracellular vesicles known to act as mediators of intercellular communications, remain unclear. Here, we investigated CX3CR1 expression on exosomes isolated from various cell types. Although we found that all the exosomes tested in our study highly expressed CX3CR1, this chemokine receptor was expressed only inside, but barely on, their source cells. Moreover, exosomal CX3CR1 was capable of binding soluble CX3CL1. Therefore, our study suggests that CX3CR1 is a novel and ligand-competent exosome receptor.
据报道，趋化因子受体CX3CR1的表达仅限于几种细胞类型，包括自然杀伤细胞、细胞毒性T细胞、单核细胞和巨噬细胞。然而，其在作为细胞间通讯介质的纳米级细胞外囊泡——外泌体上的表达和功能仍不清楚。在此，我们研究了从各种细胞类型中分离出的外泌体上CX3CR1的表达情况。尽管我们发现本研究中测试的所有外泌体都高度表达CX3CR1，但这种趋化因子受体仅在其来源细胞内部表达，而在细胞表面几乎不表达。此外，外泌体CX3CR1能够结合可溶性CX3CL1。因此，我们的研究表明CX3CR1是一种新型的、具有配体结合能力的外泌体受体。
