Department of Biosciences & Bioengineering, IIT Bombay, Mumbai, Maharashtra 400076, India.
Department of Chemical Engineering, IIT Bombay, Mumbai, Maharashtra 400076, India.
STAR Protoc. 2021 Feb 2;2(1):100310. doi: 10.1016/j.xpro.2021.100310. eCollection 2021 Mar 19.
cell migration is influenced by soluble factors as well as stiffness. Current strategies mostly account for one of these two factors to study cell migration. To understand the combinatorial effect of stiffness and chemokines on cell behavior, we have developed a microfluidic model to study stiffness-dependent chemotaxis of mesenchymal stem cells (hMSCs). A detailed description of our methodology will help researchers develop microfluidic models that combine these two factors influencing cell behavior. For complete details on the use and execution of this protocol, please refer to Saxena et al. (2018).
细胞迁移受可溶性因子和刚度的影响。目前的策略主要考虑这两个因素中的一个来研究细胞迁移。为了了解刚度和趋化因子对细胞行为的组合效应，我们开发了一种微流控模型来研究间充质干细胞（hMSCs）的刚度依赖性趋化性。我们方法的详细描述将帮助研究人员开发结合这两个影响细胞行为的因素的微流控模型。有关该方案使用和执行的完整详细信息，请参见 Saxena 等人。（2018 年）。
