四种热休克蛋白作为重组疫苗对尖吻鲈（Lates calcarifer）光体病的保护效力。

Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer).

机构信息

International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan.

Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Research Centre for Animal Biologics, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan.

出版信息

Fish Shellfish Immunol. 2021 Apr;111:179-188. doi: 10.1016/j.fsi.2021.02.002. Epub 2021 Feb 6.

DOI:10.1016/j.fsi.2021.02.002

PMID:33556554

Abstract

Photobacterium damselae subsp. piscicida (Phdp) is the causative agent of photobacteriosis in marine fish and is responsible for huge losses to marine aquaculture worldwide. Efforts have been made to develop a vaccine against this disease. Heat-shock proteins (HSPs) are a family of proteins that are ubiquitous in cellular life. Bacteria produce elevated levels of HSPs as a survival strategy when exposed to stressful environments in a host during infection. This group of proteins are also important antigens that can induce both humoral and cellular immune responses. In this study, four HSPs of Phdp, HSP90, HSP33, HSP70, and DnaJ, were selected for cloning and recombinant expression. Western blotting with rabbit anti-Phdp helped identify rHSP70 and rHSP33 as immunogenic proteins. Asian seabass (Lates calcarifer) immunised with rHSP90, rHSP33, rHSP70, and rDnaJ showed 48.28%, 62.07%, 51.72%, and 31.03% relative percent survival, respectively, after being challenged with Phdp strain AOD105021. High expression levels of immune-related genes and high antibody titres were observed in the rHSP33 group, and the sera of this group also exhibited a high level of bactericidal activity against Phdp. Collectively, our results suggest that HSP33 is a potential candidate for vaccine development against Phdp infection.

摘要

鱼类发光杆菌弱毒亚种（Phdp）是鱼类发光病的病原体，也是造成世界范围内海水养殖业重大损失的原因。目前，人们已经开展了针对该疾病的疫苗研发工作。热休克蛋白（HSPs）是一类广泛存在于细胞生命中的蛋白质。当细菌在感染宿主时暴露于应激环境中，它们会产生高水平的 HSPs 作为一种生存策略。这些蛋白质也是重要的抗原，可以诱导体液和细胞免疫反应。在这项研究中，我们选择了 Phdp 的 4 种 HSPs（HSP90、HSP33、HSP70 和 DnaJ）进行克隆和重组表达。兔抗 Phdp 的 Western blot 有助于鉴定 rHSP70 和 rHSP33 是免疫原性蛋白。用 rHSP90、rHSP33、rHSP70 和 rDnaJ 免疫亚洲石斑鱼（Lates calcarifer）后，在受到 Phdp 菌株 AOD105021 挑战时，相对存活率分别为 48.28%、62.07%、51.72%和 31.03%。在 rHSP33 组中观察到免疫相关基因的高表达水平和高抗体滴度，并且该组的血清对 Phdp 也表现出高水平的杀菌活性。总的来说，我们的结果表明 HSP33 是一种有潜力的 Phdp 感染疫苗候选物。