悬浮或贴壁细胞中的 FISH 检测

FISH in Suspension or in Adherent Cells.

机构信息

Water Research Institute C.N.R., Monterotondo, Italy.

CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal.

出版信息

Methods Mol Biol. 2021;2246:51-67. doi: 10.1007/978-1-0716-1115-9_4.

DOI:10.1007/978-1-0716-1115-9_4

PMID:33576982

Abstract

Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.

摘要

荧光原位杂交（FISH）可用于检测和计数多种样本中的微生物。通常使用与 16S 或 23S rRNA 序列互补的短寡核苷酸 DNA 探针来靶向不同的系统发育水平。本章介绍了 FISH 在混合微生物群落中聚集或悬浮细胞中的应用，特别强调了环境样本。