Asgari Fatemeh, Khosravimelal Sadjad, Koruji Morteza, Aliakbar Ahovan Zahra, Shirani Ali, Hashemi Ali, Ghasemi Hamidabadi Hatef, Chauhan Narendra Pal Singh, Moroni Lorenzo, Reis Rui L, Kundu Subhas C, Gholipourmalekabadi Mazaher
Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2021 Feb;121:111814. doi: 10.1016/j.msec.2020.111814. Epub 2020 Dec 24.
Decellularization, preservation protocol and storage time influence the biomechanical and biological properties of allografts and xenografts. Here, we examined the consequences of storage time on the antibacterial, angiogenic and biocompatibility properties of the decellularized placental sponge (DPS) in vitro and in vivo. The DPS samples were preserved for one, three and six months at -20 °C. The decellularized scaffolds showed uniform morphology with interconnected pores compared with not decellularized sponges. Storage time did not interfere with collagen and vascular endothelial growth factor contents, and cytobiocompatibility for Hu02 fibroblast cells. Chorioallantoic membrane assay and subcutaneous implantation indicated a decreased new vessel formation and neovascularization in six months DPS sample compared with other experimental groups. The number of CD4 and CD68 cells infiltrated into the six months DPS on the implanted site showed a significant increase compared with one and three months sponges. The antibacterial activities and angiogenic properties of the DPS decreased over storage time. Three months preservation at -20 °C is suggested as the optimal storage period to retain its antibacterial activity and high stimulation of new vessel formation. This storage protocol could be considered for preservation of similar decellularized placenta-derived products with the aim of retaining their biological properties.
去细胞化、保存方案和储存时间会影响同种异体移植物和异种移植物的生物力学和生物学特性。在此，我们研究了储存时间对去细胞化胎盘海绵（DPS）体外和体内抗菌、血管生成及生物相容性特性的影响。DPS样本在-20°C下保存1个月、3个月和6个月。与未去细胞化的海绵相比，去细胞化支架呈现出具有相互连通孔隙的均匀形态。储存时间并未干扰胶原蛋白和血管内皮生长因子的含量，以及对Hu02成纤维细胞的细胞生物相容性。绒毛尿囊膜试验和皮下植入表明，与其他实验组相比，6个月的DPS样本中新生血管形成和血管新生减少。与1个月和3个月的海绵相比，植入部位浸润到6个月DPS中的CD4和CD68细胞数量显著增加。DPS的抗菌活性和血管生成特性随储存时间而降低。建议在-20°C下保存3个月作为保留其抗菌活性和高刺激新生血管形成的最佳储存期。为保留其生物学特性，可考虑采用此储存方案来保存类似的去细胞化胎盘衍生产品。
