脂鲤科硬骨鱼纲红带半线脂鲤受精卵膜的超微结构

Ultrastructure of the fertilized egg envelopes in Hemigrammus erythrozonus, Characidae, Teleostei.

作者信息

Chang Byung Soo, Choi Eun-Kyung, Kim Hyun-Wook, Kim Dong Heui

机构信息

Department of Cosmetology, Hanseo University, Seosan, 31962, Korea.

College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea.

出版信息

Appl Microsc. 2019 Aug 20;49(1):8. doi: 10.1186/s42649-019-0010-8.

DOI:10.1186/s42649-019-0010-8

PMID:33580400

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7809581/

Abstract

We examined the morphology and ultrastructures of fertilized egg envelopes of glowlight tetra (Hemigrammus erythrozonus) belong to Characidae using light and electron microscopes.The fertilized eggs were spherical, transparent, demersal, adhesive, and have no oil droplet. The perivitelline space was well-developed and the micropyle was surrounded by 15-20 uplifted lines of egg envelope in a spoke like pattern. The outer surface of egg envelope was rough side with grooves. Also, the total thickness of the fertilized egg envelope was about 2.1-2.3 μm, and the fertilized egg envelope consisted of two layers, an outer adhesive electron-dense layer with grooves and three feather-like lamellae layers. Collectively, these morphological characteristics of fertilized egg and micropyle with spoke-like structure showed family Characidae specificity, and ultrastructures of outer surface and section of fertilized egg envelope showed species specificity.

摘要

我们使用光学显微镜和电子显微镜检查了脂鲤科的宝莲灯鱼（Hemigrammus erythrozonus）受精卵膜的形态和超微结构。受精卵呈球形、透明、沉性、具粘性且无油滴。卵周隙发育良好，卵孔被15 - 20条呈辐条状隆起的卵膜线所包围。卵膜外表面粗糙且有凹槽。此外，受精卵膜的总厚度约为2.1 - 2.3μm，受精卵膜由两层组成，一层是具凹槽的外粘性电子致密层和三层羽毛状薄片层。总体而言，这些具有辐条状结构的受精卵和卵孔的形态特征显示出脂鲤科的特异性，而受精卵膜外表面和切片的超微结构显示出物种特异性。