青霉素在大鼠琼脂和大脑皮层中的扩散。

Diffusion of penicillin in agar and cerebral cortex of the rat.

作者信息

Lehmenkühler A, Kersting U, Nicholson C

机构信息

Institut für Physiologie, Bereich Neurophysiologie, Münster, F.R.G.

出版信息

Brain Res. 1988 Mar 15;444(1):181-3. doi: 10.1016/0006-8993(88)90926-2.

DOI:10.1016/0006-8993(88)90926-2

PMID:3359288

Abstract

The diffusion of penicillin was studied in agar gel and the cerebral cortex of the rat using pressure microinjection and ion-selective microelectrodes selective to penicillin. From the agar measurements a free diffusion coefficient for penicillin of 3.52 +/- 0.08 (mean +/- S.E.M.) X 10(-6) cm2.s-1 for 37 degrees C was determined. The tortuosity value in the cortex was 1.62 +/- 0.03 (mean +/- S.E.M.) at the same temperature implying an apparent diffusion coefficient of 1.34 +/- 0.07 (mean +/- S.E.M.) x 10(-6) cm2.s-1. This tortuosity value means that penicillin diffuses in the cortex in a similar manner to other extracellular substances. These diffusion values clarify previous estimates and permit accurate evaluation of epilepsy models based on the application of penicillin.

摘要

使用压力微量注射和对青霉素具有选择性的离子选择性微电极，对青霉素在琼脂凝胶和大鼠大脑皮层中的扩散进行了研究。根据琼脂测量结果，确定了37℃时青霉素的自由扩散系数为3.52±0.08（平均值±标准误）×10⁻⁶ cm²·s⁻¹。在相同温度下，皮层中的曲折度值为1.62±0.03（平均值±标准误），这意味着表观扩散系数为1.34±0.07（平均值±标准误）×10⁻⁶ cm²·s⁻¹。这个曲折度值表明青霉素在皮层中的扩散方式与其他细胞外物质相似。这些扩散值澄清了先前的估计，并允许基于青霉素的应用对癫痫模型进行准确评估。