Department of Entomology and Plant Pathology, University of Arkansas, Fayetteville, AR 72701, U.S.A.
Mol Plant Microbe Interact. 2021 Jun;34(6):691-702. doi: 10.1094/MPMI-12-20-0345-R. Epub 2021 Jul 8.
Fatty acid desaturases (FADs) in plants influence levels of susceptibility to multiple stresses, including insect infestations. In this study, populations of the green peach aphid () on were reduced by mutations in three desaturases: , which encodes a chloroplastic stearoyl-[acyl-carrier-protein] 9-desaturase, and or which encode ω-3 FADs in the chloroplast and endoplasmic reticulum (ER), respectively. These data indicate that certain FADs promote susceptibility to aphids and that aphids are impacted by desaturases in both the chloroplast and ER. Aphid resistance in , , and , singly or in combination, might involve altered signaling between these subcellular compartments. C18:1 levels are depleted in , whereas C18:2 accumulation is enhanced in and . In contrast, has higher than normal C18:2 levels but also high C18:1 and low C18:0 and does not impact aphid numbers. Potentially, aphids may be influenced by the balance of multiple fatty acids (FAs) rather than by a single species, with C18:2 promoting aphid resistance and C18:1 promoting susceptibility. Although the mutant also accumulates higher-than-normal levels of C16:2, this FA does not contribute to aphid resistance because a triple mutant line that lacks detectable levels of C16:2 () retains comparable levels of aphid resistance as In addition, aphid numbers are unaffected by the mutation that inhibits C16:1 synthesis. Together, these results demonstrate that certain FADs are important susceptibility factors in plant-aphid interactions and that aphid resistance is more strongly associated with differences in C18 abundance than C16 abundance.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
脂肪酸去饱和酶（FADs）在植物中影响对多种胁迫的敏感性，包括昆虫侵害。在这项研究中，通过三个去饱和酶的突变减少了桃蚜（Myzus persicae）在 上的种群： ，编码质体硬脂酰-[酰基载体蛋白] 9-去饱和酶， 和 或 ，分别编码叶绿体和内质网（ER）中的 ω-3 FADs。这些数据表明，某些 FADs促进了对蚜虫的敏感性，并且蚜虫受到叶绿体和 ER 中 FADs的影响。 、 、 和 单独或组合的抗蚜性可能涉及这些亚细胞隔室之间信号的改变。 中 C18:1 水平耗尽，而 C18:2 积累在 和 中增强。相比之下， 具有高于正常水平的 C18:2，但也具有高 C18:1 和低 C18:0，并且不影响蚜虫数量。潜在地，蚜虫可能受到多种脂肪酸（FAs）的平衡而不是单一物种的影响，其中 C18:2 促进蚜虫抗性，而 C18:1 促进易感性。尽管 突变体也积累了高于正常水平的 C16:2，但这种 FA 不会导致蚜虫抗性，因为缺乏可检测水平的 C16:2 的三重突变体系 保留了与 相同水平的蚜虫抗性。此外，抑制 C16:1 合成的 突变对蚜虫数量没有影响。总之，这些结果表明，某些 FADs是植物-蚜虫相互作用中重要的易感性因素，并且蚜虫抗性与 C18 丰度的差异比 C16 丰度的差异更密切相关。[公式：见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
