热处理后肉毒梭菌孢子在酵母提取物琼脂和猪肉浸液琼脂中的回收率。
Recovery of spores of Clostridium botulinum in yeast extract agar and pork infusion agar after heat treatment.
作者信息
Odlaug T E, Pflug I J
出版信息
Appl Environ Microbiol. 1977 Oct;34(4):377-81. doi: 10.1128/aem.34.4.377-381.1977.
Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast extract agar, compared to the D-values for spores recovered in pork infusion agar. The z-values for strains 62A and A16037 were largest when the spores were recovered in pork infusion agar. The addition of sodium bicarbonate and sodium thioglycolate to pork infusion agar resulted in D-values for C. botulinum 62A spores similar to those for the same spores recovered in yeast extract agar. The results suggest that sodium bicarbonate and sodium thioglycolate should be added to recovery media for heated C. botulinum spores to obtain maximum plate counts.
酵母提取物琼脂、猪肉浸出液琼脂以及这些培养基的改良培养基被用于复苏经加热处理的肉毒梭菌孢子。测定了D值和z值。研究了两株A型肉毒梭菌菌株和一株B型肉毒梭菌菌株。在所有情况下，与在猪肉浸出液琼脂中复苏的孢子的D值相比，当孢子在酵母提取物琼脂中复苏时，D值最大。当孢子在猪肉浸出液琼脂中复苏时，62A菌株和A16037菌株的z值最大。向猪肉浸出液琼脂中添加碳酸氢钠和巯基乙酸钠后，肉毒梭菌62A孢子的D值与在酵母提取物琼脂中复苏的相同孢子的D值相似。结果表明，应向用于复苏经加热处理的肉毒梭菌孢子的培养基中添加碳酸氢钠和巯基乙酸钠，以获得最大的平板计数。
Zotero 插件