CRISPR/Cas9 系统在植物非生物胁迫耐受工程中的潜在应用。
Potential Application of CRISPR/Cas9 System to Engineer Abiotic Stress Tolerance in Plants.
机构信息
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan.
出版信息
Protein Pept Lett. 2021;28(8):861-877. doi: 10.2174/0929866528666210218220138.
Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review highlighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.
植物中的非生物胁迫，如盐度、干旱、重金属毒性、热和养分限制，极大地降低了全球的农业产量。转录激活子样效应物核酸酶（TALENs）和锌指核酸酶（ZFNs）等基因组编辑技术已被用于植物的基因组操作。然而，最近出现的成簇规律间隔短回文重复（CRISPR）/CRISPR 相关蛋白 9（Cas9）技术已成为植物基因组编辑中获得理想性状的一种很有前途的工具。CRISPR/Cas9 系统在培育对非生物胁迫具有更好耐受性的作物品种方面具有巨大的潜力。本综述主要介绍了 CRISPR/Cas9 系统的生物学特性及其在提高植物非生物胁迫耐受性方面的潜在应用。此外，本综述还强调了 CRISPR/Cas9 介导的基因组编辑在可持续农业方面的最新进展。
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