Li Ming, Wu Meng, Xie Hong-Ju, Liu Hong-Wei
Laser Center of Plastic Surgery and Cosmetology, The First Affiliated Hospital of Jinan University, Guangzhou City, China.
Department of Plastic Surgery, Second Affiliated Hospital of Hainan Medical College, Haikou City, China.
Wound Repair Regen. 2021 May;29(3):393-405. doi: 10.1111/wrr.12900. Epub 2021 Feb 19.
Nasal capsular contracture is a prevalent complication commonly observed after rhinoplasty. However, the mechanism underlying the pathogenesis of nasal capsular contracture is largely unclear compared to that of breast capsular contracture. This study aimed to identify the key genes implicated in nasal capsular contracture progression using RNA deep sequencing (RNA-seq). Biopsy samples were taken from Grade II to Grade IV nasal fibrous capsular tissues. The former is regarded as the relatively normal tissues and thus was set as control group, while the latter was treated as pathological group. Results from RNA-seq underwent GO enrichment and KEGG pathway analysis and subsequent verification by quantitative reverse transcriptase polymerase chain reaction and western blot assays. RNA-seq analysis showed that 3149 genes were up-regulated and 3131 genes in pathological groups compared with controls. The top 30 up-regulated genes included many chemokines (e.g., CCL18, CCL13, CCL17 and CCL8), matrix metallopeptidases (e.g., MMP9 and MMP12) and integrin proteins (e.g., ITGAM and ITGB2). GO enrichment analysis demonstrated that the up-regulated genes affected various immune functions, including immune system process, cell activation, leukocyte activation, defence response and positive regulation of immune. The down-regulated gene primary influenced muscle development and functions as well as metabolic processes. In summary, this study reveal that abnormal changes of immune functions, muscle develop and metabolic processes are probably implicated in the pathogenesis of nasal capsular contracture.
鼻包膜挛缩是隆鼻术后常见的并发症。然而，与乳房包膜挛缩相比，鼻包膜挛缩发病机制背后的原因在很大程度上尚不清楚。本研究旨在通过RNA深度测序（RNA-seq）确定与鼻包膜挛缩进展相关的关键基因。从II级至IV级鼻纤维包膜组织中获取活检样本。前者被视为相对正常的组织，因此被设为对照组，而后者被视为病理组。RNA-seq的结果进行了基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）通路分析，并随后通过定量逆转录聚合酶链反应和蛋白质免疫印迹分析进行验证。RNA-seq分析显示，与对照组相比，病理组中有3149个基因上调，3131个基因下调。上调的前30个基因包括许多趋化因子（如CCL18、CCL13、CCL17和CCL8）、基质金属蛋白酶（如MMP9和MMP12）和整合素蛋白（如ITGAM和ITGB2）。GO富集分析表明，上调的基因影响多种免疫功能，包括免疫系统过程、细胞活化、白细胞活化、防御反应和免疫的正调控。下调的基因主要影响肌肉发育和功能以及代谢过程。总之，本研究表明免疫功能、肌肉发育和代谢过程的异常变化可能与鼻包膜挛缩的发病机制有关。
