National Reference Laboratory for meningococci, National Centre for Microbiology, Instituto de Salud Carlos III, Ctra, Majadahonda-Pozuelo, Km2., 28220 Majadahonda, Madrid, Spain.
National Reference Laboratory for meningococci, National Centre for Microbiology, Instituto de Salud Carlos III, Ctra, Majadahonda-Pozuelo, Km2., 28220 Majadahonda, Madrid, Spain.
J Infect. 2021 Apr;82(4):37-44. doi: 10.1016/j.jinf.2021.01.030. Epub 2021 Feb 18.
Studies of meningococcal genetic population structure, including the potential associations between surface proteins variants and clonal complexes, are important to understand how new protein MenB vaccines might impact in specific scenarios. With the aim to analyze the diversity of Spanish invasive MenB strains, and genetic variability of the fHbp vaccine antigen, all MenB isolates received at National Reference Laboratory (NRL) from 2015 to 2018 were molecularly characterized.
108, 103, 87 and 112 invasive MenB strains isolated during 2015-2018, respectively, were received at NRL. The strains were whole genome sequenced, and porA, fetA, MLST and fHbp variability was analyzed. Potential impact on MenB vaccines coverage was also assessed.
A total of 42, 38 and 3 different FHbp subfamily A, B and A/B hybrid peptides, respectively, were found. FHbp subfamily A peptides were harboured by most of the strains (65.9%), being the most prevalent peptide 45 which was associated with genosubtype 22,14 and cc213. FHbp subfamily B peptides were harboured by 32.4% of the strains, and 6 strains harbouring subfamily A/B hybrid peptides were also found. The 64.15% of the strains showed FHbp variants "exact-match" or "cross-reactive" to the FHbp variants included in rLP2086 vaccine according to hSBA assays in the rLP2086 clinical development, and 15.85% showed FHbp peptides defined as predictors of FHbp-coverage for 4CMenB vaccine by gMATS.
Due to invasive meningococcal strains temporal variability (eg prevalence of the cc213 increased from 3.6% in 2007 to 33% in 2018) affecting to the presence and distribution of the vaccine antigens, continuous detailed meningococcal surveillance and monitoring of the vaccine antigens is needed to determine the degree and durability of coverage provided by these protein vaccine.
分析西班牙侵袭性 B 群脑膜炎奈瑟菌（MenB）分离株的多样性以及 fHbp 疫苗抗原的遗传变异性，对 2015 年至 2018 年期间国家参考实验室（NRL）收到的所有 MenB 分离株进行分子特征分析。
2015 年至 2018 年期间，NRL 共收到 108、103、87 和 112 株侵袭性 MenB 分离株。对这些菌株进行全基因组测序，并分析 porA、fetA、MLST 和 fHbp 的变异性。同时评估对 MenB 疫苗覆盖率的潜在影响。
共发现 42、38 和 3 种不同的 fHbp 亚家族 A、B 和 A/B 杂合肽，分别为。大多数菌株携带 fHbp 亚家族 A 肽（65.9%），最常见的肽 45 与基因亚型 22、14 和 cc213 相关。32.4%的菌株携带 fHbp 亚家族 B 肽，还发现 6 株携带 fHbp 亚家族 A/B 杂合肽。根据 rLP2086 临床试验中 hSBA 检测，64.15%的菌株的 fHbp 变异与 rLP2086 疫苗中包含的 fHbp 变异具有“完全匹配”或“交叉反应性”，15.85%的菌株显示 fHbp 肽被定义为 4CMenB 疫苗预测 fHbp 覆盖率的指标。
由于侵袭性脑膜炎奈瑟菌菌株的时间变异性（例如，cc213 的流行率从 2007 年的 3.6%增加到 2018 年的 33%）影响了疫苗抗原的存在和分布，需要对这些蛋白疫苗进行持续详细的脑膜炎监测和监测，以确定这些蛋白疫苗提供的覆盖范围的程度和持久性。
Zotero 插件