Kamimura Seiichiro, Kitamura Yoshiaki, Fujii Tatsuya, Okamoto Kentaro, Sanada Nanae, Okajima Natsuki, Wakugawa Tomoharu, Fukui Hiroyuki, Mizuguchi Hiroyuki, Takeda Noriaki
Department of Otolaryngology Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima Japan.
Department of Molecular Pharmacology Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima Japan.
Laryngoscope Investig Otolaryngol. 2021 Jan 8;6(1):34-41. doi: 10.1002/lio2.518. eCollection 2021 Feb.
Phototherapy with narrow-band ultraviolet B (narrow-band UVB) is clinically effective treatment for atopic dermatitis. In the present study, we examined the effects of intranasal irradiation with narrow-band UVB on nasal symptom, upregulation of histamine H receptor (H1R) gene expression and induction of DNA damage in the nasal mucosa of allergic rhinitis (AR) model rat.
AR model rats were intranasally irradiated with 310 nm of narrow-band UVB. Nasal mucosal levels of H1R mRNA were measured using real-time quantitative reverse transcriptase (RT)-PCR. DNA damage was evaluated using cyclobutane pyrimidine dimer (CPD) immunostaining.
In toluene 2,4-diisocyanate (TDI)-sensitized rats, TDI provoked sneezes and H1R gene expression in the nasal mucosa. Intranasal pre-irradiation with 310 nm narrow-band UVB at doses of 600 and 1400, but not 200 mJ/cm significantly inhibited the number of sneezes and upregulation of H1R gene expression provoked by TDI. CPD-positive cells appeared in the nasal mucosa after intranasal narrow-band UVB irradiation at a dose of 1400, but not 200 and 600 mJ/cm. The suppression of TDI-provoked sneezes and upregulation of H1R gene expression lasted 24 hours, but not 48 hours, after narrow-band UVB irradiation with a dose of 600 mJ/cm.
Intranasal pre-irradiation with narrow-band UVB dose-dependently inhibited sneezes and upregulation of H1R gene expression of the nasal mucosa in AR model rats, suggesting that the inhibition of nasal upregulation of H1R gene expression suppressed nasal symptom. The suppression after narrow-band UVB irradiation at a dose of 600 mJ/cm was reversible without induction of DNA damage. These findings indicated that low-dose narrow-band UVB phototherapy could be effectively and safely used for AR treatment in a clinical setting.
NA.
窄谱中波紫外线（窄谱UVB）光疗是治疗特应性皮炎的临床有效方法。在本研究中，我们检测了窄谱UVB鼻腔内照射对变应性鼻炎（AR）模型大鼠鼻腔症状、组胺H受体（H1R）基因表达上调以及鼻腔黏膜DNA损伤诱导的影响。
对AR模型大鼠进行310nm窄谱UVB鼻腔内照射。使用实时定量逆转录酶（RT）-PCR检测鼻腔黏膜中H1R mRNA水平。使用环丁烷嘧啶二聚体（CPD）免疫染色评估DNA损伤。
在2,4-二异氰酸甲苯酯（TDI）致敏的大鼠中，TDI诱发了打喷嚏以及鼻腔黏膜中H1R基因表达。剂量为600和1400mJ/cm而非200mJ/cm的310nm窄谱UVB鼻腔内预照射显著抑制了TDI诱发的打喷嚏次数以及H1R基因表达上调。剂量为1400mJ/cm而非200和600mJ/cm的窄谱UVB鼻腔内照射后，鼻腔黏膜中出现了CPD阳性细胞。剂量为600mJ/cm的窄谱UVB照射后，TDI诱发的打喷嚏抑制以及H1R基因表达上调持续24小时，但未持续48小时。
窄谱UVB鼻腔内预照射剂量依赖性地抑制了AR模型大鼠鼻腔黏膜打喷嚏以及H1R基因表达上调，提示抑制鼻腔黏膜中H1R基因表达上调可减轻鼻腔症状。600mJ/cm剂量的窄谱UVB照射后的抑制作用是可逆的，且未诱导DNA损伤。这些发现表明低剂量窄谱UVB光疗在临床环境中可有效且安全地用于AR治疗。
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