从手术部位感染分离的细菌的抗菌药敏模式：重点关注金黄色葡萄球菌；伊朗西南部亚苏季市。

Antimicrobial Susceptibility Pattern in the Bacteria Isolated from Surgical Site Infection: Emphasis on Staphylococcus Aureus; Yasuj City, Southwest Iran.

出版信息

Clin Lab. 2021 Feb 1;67(2). doi: 10.7754/Clin.Lab.2020.200530.

DOI:10.7754/Clin.Lab.2020.200530

Abstract

BACKGROUND

Surgical site infections (SSIs) in surgical wards remains the most common cause of postoperative complications and realistically is the third most common origin of healthcare-related conditions. Staphylococcus aureus is undoubtedly the most common bacteria causing SSIs. The current study aimed at investigating the antimicrobial susceptibility pattern in bacteria isolated from SSIs, evaluation of tetracycline resistance genes, and SCCmec typing in S. aureus isolates isolated from patients with SSIs from 2018 to 2019 in Yasuj, Kohgiluyeh, and Boyer-Ahmad Province, Iran.

METHODS

This study diligently investigated 240 potential patients. Antimicrobial susceptibility testing was performed properly by the disk diffusion method. For the final confirmation of isolated bacteria, PCR was used. The presence of tet genes and SCCmec typing was carried out by multiplex PCR.

RESULTS

The results showed that the most common isolated pathogens included S. aureus, E. coli, P. aeruginosa, Coagulase-negative Staphylococci, and K. pneumonia in 58.8%, 19.8%, 9.2%, 6.8% and 5.4% of cases, respectively. The majority of the Gram positive isolates were resistant against penicillin (86%) and Gram negative were resistant against ciprofloxacin (75.6%). In isolates of Staphylococcus aureus, the mecA gene was detected in 63.6% of isolates. The predominant SCCmec types were type III (59.1%) and type I (18.4%). The tetK and tetM genes were detected in 80.7% and 71.9% of the S. aureus isolates, respectively. There was a statistically significant difference between tet genes (tetK and tetM) from the viewpoint of resistance to tetracycline (p = 0.024).

CONCLUSIONS

According to the results of the current study, it is recommended to administer vancomycin, amikacin, and imipenem in Yasuj to treat SSIs.

摘要

背景

外科病房的手术部位感染（SSI）仍然是术后并发症的最常见原因，实际上是医疗相关疾病的第三大常见来源。金黄色葡萄球菌无疑是引起 SSI 的最常见细菌。本研究旨在调查 2018 年至 2019 年伊朗亚苏季、戈尔甘和博耶-艾哈迈德省 SSI 患者分离的 SSI 分离细菌的抗菌药物敏感性模式，评估四环素耐药基因，以及金黄色葡萄球菌分离株的 SCCmec 分型。

方法

本研究认真调查了 240 名潜在患者。通过纸片扩散法进行了适当的抗菌药物敏感性测试。为了最终确认分离细菌，使用 PCR。通过多重 PCR 检测 tet 基因和 SCCmec 分型。

结果

结果显示，最常见的分离病原体包括金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、凝固酶阴性葡萄球菌和肺炎克雷伯菌，分别占 58.8%、19.8%、9.2%、6.8%和 5.4%。大多数革兰氏阳性分离株对青霉素（86%）耐药，革兰氏阴性分离株对环丙沙星（75.6%）耐药。在金黄色葡萄球菌分离株中，mecA 基因在 63.6%的分离株中被检测到。主要的 SCCmec 类型为 III 型（59.1%）和 I 型（18.4%）。在 80.7%和 71.9%的金黄色葡萄球菌分离株中分别检测到 tetK 和 tetM 基因。从对四环素的耐药性（p=0.024）来看，tet 基因（tetK 和 tetM）存在统计学显著差异。