建立并应用实时 RT-PCR 方法快速检测 H2 亚型禽流感病毒。
Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses.
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, and National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
J Vet Diagn Invest. 2021 May;33(3):577-581. doi: 10.1177/1040638721994810. Epub 2021 Feb 22.
Abstract
The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.
摘要
禽流感病毒（avian IAV）的 H2 亚型一直在禽类中传播，并且有可能感染人类。因此，建立一种快速检测这种亚型的方法至关重要。我们开发了一种 TaqMan 小沟结合物实时 RT-PCR 检测方法，该方法使用基于基质和血凝素基因保守序列的探针和引物。该检测方法的检测限低至每个反应 50%的鸡胚半数感染剂量（EID）/毫升。该检测方法特异性强、灵敏度高、快速，可用于检测禽源 IAV H2 亚型。
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