Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 11529, Taiwan.
Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan.
ISME J. 2021 Jun;15(6):1858-1861. doi: 10.1038/s41396-020-00871-0. Epub 2021 Feb 22.
Bacteria and fungi secrete many natural products that inhibit each other's growth and development. The dynamic changes in secreted metabolites that occur during interactions between bacteria and fungi are complicated. Pyochelin is a siderophore produced by many Pseudomonas and Burkholderia species that induces systemic resistance in plants and has been identified as an antifungal agent. Through imaging mass spectrometry and metabolomics analysis, we found that Phellinus noxius, a plant pathogen, can modify pyochelin and ent-pyochelin to an esterification product, resulting in reduced iron-chelation and loss of antifungal activity. We also observed that dehydroergosterol peroxide, the fungal metabolite, is only accumulated in the presence of pyochelin produced through bacteria-fungi interactions. For the first time, we show the fungal transformation of pyochelin in the microbial interaction. Our findings highlight the importance of understanding the dynamic changes of metabolites in microbial interactions and their influences on microbial communities.
细菌和真菌分泌许多相互抑制生长和发育的天然产物。细菌和真菌相互作用过程中分泌代谢物的动态变化非常复杂。焦脱镁叶绿酸是许多假单胞菌属和伯克霍尔德菌属产生的一种铁载体，它能诱导植物产生系统抗性，已被鉴定为一种抗真菌剂。通过成像质谱和代谢组学分析，我们发现植物病原体鲍氏层孔菌能够将焦脱镁叶绿酸和表焦脱镁叶绿酸修饰成酯化产物，导致铁螯合能力降低和抗真菌活性丧失。我们还观察到真菌代谢产物去氢麦角甾醇过氧化物只有在细菌-真菌相互作用产生的焦脱镁叶绿酸存在的情况下才会积累。我们首次展示了微生物相互作用中焦脱镁叶绿酸的真菌转化。我们的研究结果强调了理解微生物相互作用中代谢物的动态变化及其对微生物群落影响的重要性。
