College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, People's Republic of China.
Hubei Key Laboratory for Efficient Utilization and Agglomeration of metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, People's Republic of China.
Environ Technol. 2022 Jul;43(17):2697-2711. doi: 10.1080/09593330.2021.1895323. Epub 2021 Mar 11.
In this study, an amino-functionalized magnetic silica microsphere material (FeO-SiO-NH) was prepared. Using glutaraldehyde as a cross-linking agent, laccase was adsorbed-covalently bonded and immobilized on the material to prepare Laccase @ FeO-SiO. In addition, the materials were characterized and analysed by SEM, TEM, XRD, FT-IR and VSM. Finally, the thermal inactivation dynamics of immobilized laccase in polar/non-polar/toxic systems and the adsorption and degradation of 2,4-DCP were studied. The results showed that Laccase @ FeO-SiO under the optimal conditions (pH 6, temperature 65°C, initial concentration of 2,4-DCP 10 mg/L), the removal rate was as high as 81.6%. Moreover, compared with free laccase, immobilized laccase had good tolerance under low pH and high-temperature conditions, and storage stability was also greatly improved. After repeated use for 7 times, Laccase @ FeO-SiO can still maintain 59% removal rate of 2,4-DCP, which gives it the potential for industrial applications.
在本研究中，制备了一种氨基功能化磁性硅球材料（FeO-SiO-NH）。使用戊二醛作为交联剂，将漆酶吸附-共价键合固定在材料上，制备漆酶@FeO-SiO。此外，通过 SEM、TEM、XRD、FT-IR 和 VSM 对材料进行了表征和分析。最后，研究了固定化漆酶在极性/非极性/有毒体系中的热失活动力学以及 2,4-DCP 的吸附和降解。结果表明，在最优条件下（pH 6，温度 65°C，2,4-DCP 初始浓度 10 mg/L），Laccase@FeO-SiO 的去除率高达 81.6%。此外，与游离漆酶相比，固定化漆酶在低 pH 和高温条件下具有良好的耐受性，储存稳定性也得到了极大提高。重复使用 7 次后，Laccase@FeO-SiO 仍能保持 2,4-DCP 80%的去除率，这使其具有工业应用的潜力。
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