Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Virology and Cancer Pathobiology Research Center, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Microbiology, Pathwest Laboratory Medicine, Perth, Australia; School of Pharmacy & Biomedical Science, Curtin University, Perth, Australia.
Clin Immunol. 2021 May;226:108696. doi: 10.1016/j.clim.2021.108696. Epub 2021 Feb 20.
Cytomegalovirus (CMV) affects γδ T-cell profiles in healthy individuals and transplant recipients, but the effects of HIV and CMV have not been distinguished in HIV patients. CMV-seropositive Indonesian HIV patients (n = 40) were studied before ART and after six months, alongside healthy controls (n = 20). 50% of patients started ART with detectable CMV DNA. Proportions of Vδ2 γδ T-cells were high in patients and declined on ART, whilst proportions of Vδ2 γδ T-cells were uniformly low, and correlated inversely with levels of CMV DNA and CMV-reactive antibody. Residual Vδ2 cells were enriched for markers of terminal differentiation, but this did not associate with CMV metrics. Patients with CMV DNA at baseline showed a direct correlation between CMV reactive-antibody and CD8 γδ T-cells. Our data are consistent with a role for CMV in the depletion of Vδ2 γδ T-cells in HIV patients beginning ART, with no consistent evidence of a role for CMV in γδ T-cell activation or differentiation.
巨细胞病毒 (CMV) 会影响健康个体和移植受者的 γδ T 细胞谱，但在 HIV 患者中尚未区分 HIV 和 CMV 的影响。研究了 CMV 阳性印度尼西亚 HIV 患者（n=40）在开始 ART 前和 6 个月后的情况，并与健康对照组（n=20）进行了比较。50%的患者开始 ART 时可检测到 CMV DNA。患者的 Vδ2 γδ T 细胞比例较高，并在 ART 后下降，而 Vδ2 γδ T 细胞比例均匀较低，与 CMV DNA 和 CMV 反应性抗体水平呈负相关。残留的 Vδ2 细胞富含终末分化标志物，但与 CMV 指标无关。基线时 CMV DNA 阳性的患者 CMV 反应性抗体与 CD8 γδ T 细胞之间存在直接相关性。我们的数据与 CMV 在开始 ART 的 HIV 患者中耗竭 Vδ2 γδ T 细胞中起作用的观点一致，但没有一致的证据表明 CMV 在 γδ T 细胞激活或分化中起作用。
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