5-羟色氨酸微生物合成的研究进展。

Liu Xin-Xin, Zhang Bin, Ai Lian-Zhong

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.

College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.

Front Bioeng Biotechnol. 2021 Feb 3;9:624503. doi: 10.3389/fbioe.2021.624503. eCollection 2021.

5-Hydroxytryptophan (5-HTP) plays an important role in the regulation of emotion, behavior, sleep, pain, body temperature, and other physiological functions. It is used in the treatment of depression, insomnia, migraine, and other diseases. Due to a lack of effective biosynthesis methods, 5-HTP is mainly obtained by natural extraction, which has been unable to meet the needs of the market. Through the directed evolution of enzymes and the introduction of substrate supply pathways, 5-HTP biosynthesis and yield increase have been realized. This review provides examples that illustrate the production mode of 5-HTP and the latest progress in microbial synthesis.

5-羟色氨酸（5-HTP）在情绪、行为、睡眠、疼痛、体温及其他生理功能的调节中发挥着重要作用。它被用于治疗抑郁症、失眠、偏头痛及其他疾病。由于缺乏有效的生物合成方法，5-HTP主要通过天然提取获得，这已无法满足市场需求。通过酶的定向进化和引入底物供应途径，实现了5-HTP的生物合成及产量增加。本文综述提供了实例，阐明了5-HTP的生产模式及微生物合成的最新进展。