College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan.
Experimental Station for Medicinal Plant Research, School of Pharmacy and Pharmaceutical Science, University of Toyama, 2630 Sugitani, Toyama, Toyama, 930-0194, Japan.
J Nat Med. 2021 Jun;75(3):699-706. doi: 10.1007/s11418-021-01488-z. Epub 2021 Feb 25.
In order to differentiate among Valeriana fauriei Briq. and other Eurasian medicinal valerian (V. dioica L., V. hardwickii Wall., V. jatamansi Jones, and V. officinalis L.), we attempted to establish DNA markers. DNA sequences for the psbA-trnH intergenic spacer region of chloroplast DNA (psbA-trnH) and 18S ribosomal RNA, internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA, internal transcribed spacer 2 (ITS2), and 28S ribosomal RNA of nuclear DNA in V. fauriei and other Eurasian medicinal valerian were compared. Using partial sequences of psbA-trnH (nucleotide positions 1-75 from the 5' end of the intergenic spacer region), V. fauriei and other Eurasian medicinal valerian could be correctly identified to the species level. In addition, the partial sequences of psbA-trnH in V. fauriei contained five different haplotypes, and it was possible to distinguish the origins of valerian from Japan and Eurasia (China and Korea). On the other hand, individuals had heterogeneous sequences of ITS1 and ITS2, making it impossible to use direct sequencing and DNA markers of ITS1 and ITS2 to distinguish species and origins of V. fauriei and other Eurasian medicinal valerian.
为了区分欧洲药用缬草（V. dioica L.、V. hardwickii Wall.、V. jatamansi Jones 和 V. officinalis L.）和法氏缬草（Valeriana fauriei Briq.），我们试图建立 DNA 标记。比较了法氏缬草和其他欧洲药用缬草的叶绿体 DNA（psbA-trnH）和核 DNA 的 psbA-trnH 基因间隔区、18S 核糖体 RNA、内部转录间隔区 1（ITS1）、5.8S 核糖体 RNA、内部转录间隔区 2（ITS2）和 28S 核糖体 RNA 的部分序列。使用 psbA-trnH（基因间隔区 5'端的核苷酸位置 1-75）的部分序列，可以正确鉴定法氏缬草和其他欧洲药用缬草到种的水平。此外，法氏缬草的 psbA-trnH 部分序列包含五个不同的单倍型，并且可以区分来自日本和欧亚大陆（中国和韩国）的缬草起源。另一方面，个体的 ITS1 和 ITS2 序列存在异质性，因此无法使用 ITS1 和 ITS2 的直接测序和 DNA 标记来区分法氏缬草和其他欧洲药用缬草的种和起源。
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