噬菌体T4尾管组装所需的三个基因的结构。

The structure of three bacteriophage T4 genes required for tail-tube assembly.

作者信息

Ishimoto L K, Ishimoto K S, Cascino A, Cipollaro M, Eiserling F A

机构信息

Department of Microbiology, University of California, Los Angeles 90024.

出版信息

Virology. 1988 May;164(1):81-90. doi: 10.1016/0042-6822(88)90622-8.

DOI:10.1016/0042-6822(88)90622-8

PMID:3363870

Abstract

Three different protein molecules copurify with T4 tail tubes after the tubes are released from the baseplate by guanidine hydrochloride treatment. These tube-associated proteins (TAPs) are the products of genes 29, 48, and 54. To further investigate the structural roles that these proteins may play in T4 tail assembly we have cloned and sequenced the genes coding for these proteins and have deduced their predicted amino acid sequences. The sequence data reveal a region of amino acid sequence similarity between gp54 and the T4 tail-tube structural protein, gp19. We believe that this region of similarity is significant and consistent with the role gp54 may play in initiating T4 tail-tube polymerization.

摘要

用盐酸胍处理从基板释放出尾管后，有三种不同的蛋白质分子与T4尾管共纯化。这些与尾管相关的蛋白质（TAPs）是基因29、48和54的产物。为了进一步研究这些蛋白质在T4尾装配中可能发挥的结构作用，我们克隆并测序了编码这些蛋白质的基因，并推导了它们的预测氨基酸序列。序列数据揭示了gp54与T4尾管结构蛋白gp19之间的氨基酸序列相似区域。我们认为这个相似区域很重要，并且与gp54在启动T4尾管聚合中可能发挥的作用一致。