Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38089 San Michele all'Adige, Italy; Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38089 San Michele all'Adige, Italy.
Bi-PA nv, Technologielaan 7, 1840 Londerzeel, Belgium.
Microbiol Res. 2021 Jun;247:126724. doi: 10.1016/j.micres.2021.126724. Epub 2021 Feb 18.
Tagatose is a rare sugar metabolised by a limited number of microorganisms that inhibits a large spectrum of phytopathogens. In particular, tagatose inhibited Phytophthora infestans growth and negatively affected mitochondrial processes. However, the possible effects of tagatose on P. infestans metabolism have not yet been investigated. The aim of this study was to analyse the impact of this rare sugar on the sugar metabolism in P. infestans, in order to better understand its mode of action. Tagatose inhibited the growth of P. infestans with a precise reprogramming of the carbohydrate metabolism that involved a decrease of glucose, glucose-1-phosphate and mannose content and β-glucosidase activity. The combination of tagatose with common sugars led to three different responses and highlighted antagonistic interactions. In particular, glucose partially attenuated the inhibitory effects of tagatose, while fructose fully impaired tagatose-mediated growth inhibition and metabolite changes. Moreover, sucrose did not attenuate tagatose effects, suggesting that the inhibition of sucrose catabolism and the alteration of glucose-related pathways contributed to the growth inhibition caused by tagatose to P. infestans. The interactions of tagatose with the common sugar metabolism were found to be a key mode of action against P. infestans growth, which may represent the basis for the further development of tagatose as an eco-friendly fungicide.
塔格糖是一种稀有糖，只有少数微生物能够代谢，它能抑制多种植物病原体。特别是，塔格糖抑制了马铃薯晚疫病菌的生长，并对线粒体过程产生负面影响。然而，塔格糖对马铃薯晚疫病菌代谢的可能影响尚未得到研究。本研究的目的是分析这种稀有糖对马铃薯晚疫病菌糖代谢的影响，以更好地了解其作用模式。塔格糖抑制了马铃薯晚疫病菌的生长，对碳水化合物代谢进行了精确的重编程，涉及葡萄糖、葡萄糖-1-磷酸和甘露糖含量以及β-葡萄糖苷酶活性的降低。塔格糖与常见糖的组合导致了三种不同的反应，并突出了拮抗相互作用。特别是，葡萄糖部分减弱了塔格糖的抑制作用，而果糖则完全损害了塔格糖介导的生长抑制和代谢物变化。此外，蔗糖并没有减弱塔格糖的作用，这表明蔗糖分解代谢的抑制和葡萄糖相关途径的改变有助于塔格糖对马铃薯晚疫病菌生长的抑制。塔格糖与常见糖代谢的相互作用被发现是抑制马铃薯晚疫病菌生长的关键作用模式，这可能为进一步开发塔格糖作为环保型杀菌剂提供基础。
